FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14011660
Sequences flagged as poor quality0
Sequence length37
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG2682611.9145554488190548No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA2488071.7757139411033382No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG2287791.6327758452603047No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1846891.318109346073199No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA1652311.179239290705027No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT1340580.9567602982087775No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG1301450.928833557194508No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1220550.8710959301039277No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG938810.6700205400359415No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG927770.6621413879583147No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGG893880.6379543894156724No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT714490.509925305067351No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG685960.4895636919537014No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGTGG678190.4840183104642848No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA658690.4701013298923896No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGG599020.42751536934239054No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG584660.41726676211098473No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG555180.39622714225152483No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG551200.3933866508322354No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG528680.37731432249997504No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG510470.3643180037197591No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA500230.3570098046912357No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG477720.34094461327208914No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG462020.3297396596834351No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG448530.3201119638929292No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAT447920.3196766121929878No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG442870.3160724710705227No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT426830.30462486243599973No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGG422740.30170586497245866No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT411850.29393376659153875No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG408770.2917355973524907No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA387740.27672666907418536No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT386590.27590592406609926No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA369510.26371607646774187No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG359020.2562294546113737No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGA356620.2545165954640635No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATT342420.24438217884247834No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG332980.23764493286305832No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA331830.23682418785497222No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC328320.23431913135203108No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG325930.2326134091178347No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG318880.2275818853726111No Hit
CTGGACGCGAGCCGGGCCCTTCCCGTGGATTGGAATT305300.21788995736408104No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT296600.2116808429550817No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG279230.19928402487642435No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTGGA277640.19814925569133138No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT273680.19532303809826956No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG269710.192489683592094No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG268070.19131922984143207No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA261060.18631625374866362No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTC260080.18561683626351197No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAA255100.18206265353284337No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG254030.18129900382966757No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA250040.17845137549726442No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT247980.1769811713958232No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG239540.17095761672778242No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA231440.1651767171056106No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA231190.1649982942777658No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT229110.16351381635009699No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA228430.1630285062583591No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG227680.1624932377748247No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA225840.16118004576188688No Hit
GAGAAAGCTCACAAGAACTGCTAACTTGGAATTCTCG222110.15851797717044233No Hit
GTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTC217310.15509225887582198No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG216630.1546069487840841No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACT202180.14429410933465414No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTG195700.1396693896369167No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC190440.1359153733390619No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG188180.1343024309753448No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG186280.13294641748372427No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCT180910.12911389514161778No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC179860.12836451926466957No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG168880.12052818866572555No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG168860.12051391483949797No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT168630.12034976583788073No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGG167920.11984304500680149No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA165910.11840852547092921No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG161450.11522546222217782No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTG155470.11095758818012998No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT154290.11011543243270247No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTG153680.10968008073276114No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTC144600.10319976362543766No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG143210.10220773270262053No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGGAA140970.10060906416513105No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGA140590.10033786146680694No Hit
CTGCATCCACTGATAGACCTTGAACTGGAATTCTCGG140400.10020226011764487No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)