FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22611252
Sequences flagged as poor quality0
Sequence length37
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG4263361.8855037306204894No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG2396321.0597909394844656No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG2394031.0587781693822174No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG2158550.9546353293484147No Hit
CCCCGGGGAGCCCGGCGGGCTGGAATTCTCGGGTGCC2001270.8850770404044854No Hit
ACGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAA1660680.7344484949351765No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT1608720.7114687855409333RNA PCR Primer, Index 1 (100% over 25bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA1603050.7089611844580742No Hit
GCCCCGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAG1599550.7074132825550747No Hit
CCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGC1471010.6505654795232038No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA1379830.6102404236616353No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT1254110.5546397873058952No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT1243870.5501110685954055No Hit
GCCCCGCCGGGGTCGGCCTGGAATTCTCGGGTGCCAA1220310.5396914774997864No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG998100.44141739696678445No Hit
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG963310.42603125205097003No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGGAATTCTC936670.4142495072807114No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT875430.3871656465550868No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG849220.3755740725900539No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA830600.3673392344660968RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG817930.3617358295772388No Hit
CGGCTCCGGGACTGGAATTCTCGGGTGCCAAGGAACT811770.3590115222279598RNA PCR Primer, Index 1 (100% over 25bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTC801170.35432359075030434No Hit
CCGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAGGAA782280.345969343050973RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA774310.342444549289No Hit
CCCGCCGGGTCCTGGAATTCTCGGGTGCCAAGGAACT750810.33205149365457515RNA PCR Primer, Index 1 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG735150.32512573828286906No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG708460.31332188062828187No Hit
CCCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA685140.3030084313774399RNA PCR Primer, Index 1 (100% over 28bp)
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCA670970.296741639958725No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGA631530.2792989968003541No Hit
CCCGGTCAGCCCCTCTCCGGCCCCGTGGAATTCTCGG617550.27311623434208776No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC615710.2723024801987966RNA PCR Primer, Index 1 (100% over 24bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG606510.2682337094823409No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG599410.2650936799076849No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC576760.25507654330684565RNA PCR Primer, Index 1 (100% over 26bp)
GCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC551370.2438476206448011RNA PCR Primer, Index 1 (100% over 24bp)
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC534300.2362982819350295RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG530220.23449387057381874No Hit
CGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAG527130.23312729432231352No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG510100.22559564592000478No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG507440.2244192404737252No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG491080.2171839047214192No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA459760.20333239397800706No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT458910.20295647494442148No Hit
CCCCCGGGGAGCCCGGCGGGCTGGAATTCTCGGGTGC452630.2001790966727539No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG447380.1978572438182547No Hit
CCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAACTC443530.19615455172495533RNA PCR Primer, Index 1 (100% over 26bp)
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGG440700.19490296247195865No Hit
GCCGGCGGGAGCCCCGGGGATGGAATTCTCGGGTGCC433320.19163910074506268No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGGAATTCT433190.19158160724580842No Hit
CCGCCGGGGTCGGCCCTGGAATTCTCGGGTGCCAAGG427070.18887499020399226Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAAC418670.18516002563679357RNA PCR Primer, Index 1 (100% over 24bp)
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATGG412880.18259935363154592No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG406120.17960969167032415No Hit
CCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA395670.17498809884565436RNA PCR Primer, Index 1 (100% over 28bp)
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC389120.17209131099861255No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATGGAAT386860.17109180862696147No Hit
CCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA386520.17094144101352726No Hit
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAA381020.16850902373738524RNA PCR Primer, Index 1 (100% over 23bp)
CCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCC370860.16401568564182115No Hit
GCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG370690.16394050183510406RNA PCR Primer, Index 1 (100% over 29bp)
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC366280.16199014543732473No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG362370.16026091788283106No Hit
CCCGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAGGA353800.15647076950891528RNA PCR Primer, Index 1 (100% over 22bp)
CCGGTCAGCCCCTCTCCGGCCCCGTGGAATTCTCGGG349880.15473711937755594No Hit
CCGCCGGGGTCTGGAATTCTCGGGTGCCAAGGAACTC344070.15216760221857684RNA PCR Primer, Index 1 (100% over 26bp)
CCGCCGGGGTCGGCCTGGAATTCTCGGGTGCCAAGGA335230.14825804426928682RNA PCR Primer, Index 1 (100% over 22bp)
CCCCGCCGGGGTCGGCCTGGAATTCTCGGGTGCCAAG331140.14644921033121033No Hit
CCCGTGGATCGCCCCAGCTGTGGAATTCTCGGGTGCC329940.1459185011073248No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG326610.14444578301104247No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT326230.1442777250901454No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG324660.1435833805222285No Hit
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTC318260.14075293132817238RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG314980.1393023261162186No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC307980.1362065223102197RNA PCR Primer, Index 1 (100% over 31bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCT304980.1348797492505059No Hit
CGGCCCTGGCGGAGCTGGAATTCTCGGGTGCCAAGGA302800.13391562749378053RNA PCR Primer, Index 1 (100% over 22bp)
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA302410.13374314699601775No Hit
TCTGGACGCGAGCCGGGCCCTTGGAATTCTCGGGTGC299130.13229254178406397No Hit
CCGCGAGGGGGGCTGGAATTCTCGGGTGCCAAGGAAC298410.13197411624973265RNA PCR Primer, Index 1 (100% over 24bp)
CGCGCCGGGCCCGGGTCTTCCCGGATGGAATTCTCGG297630.13162915525420707No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA290240.12836087095044538No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG286870.1268704625467002No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT286590.12674663039446024No Hit
CCCGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAG285190.12612746963326046RNA PCR Primer, Index 1 (100% over 29bp)
CGGCCGGGCGCGACTGGAATTCTCGGGTGCCAAGGAA281200.1243628614638411RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC280100.12387637800861272RNA PCR Primer, Index 9 (100% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTC279050.12341200743771286No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG277640.12278842409964737No Hit
CCCCGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAGG275990.12205869891680479Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTC273940.12115207065933369No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA267610.11835257950333755No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA267580.1183393117727404No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA267350.11823759250482901No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTC266880.11802973139214053No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA266130.11769803812721207RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG257670.11395653809881913No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGATGGAATT257470.11386808656150486No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA257060.11368676091001065No Hit
CGACGGGCGCTGGAATTCTCGGGTGCCAAGGAACTCC256560.11346563206672501RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAA252000.11144893701596002No Hit
GCCCTCGGCCTGGAATTCTCGGGTGCCAAGGAACTCC251880.11139586609357148RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT249890.11051577329729463No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG248880.11006909303385767No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG247860.10961799019355495No Hit
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC240460.10634528331292757RNA PCR Primer, Index 1 (100% over 27bp)
CCCGCCGGGCATGGAATTCTCGGGTGCCAAGGAACTC239450.10589860304949059RNA PCR Primer, Index 1 (100% over 26bp)
CCGCCGGGCATGGAATTCTCGGGTGCCAAGGAACTCC237990.10525290682709652RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG236910.10477526852559955RNA PCR Primer, Index 1 (100% over 29bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA236410.10455413968231392RNA PCR Primer, Index 7 (97% over 35bp)
CCCGTGGATCGCCCCAGCTGCTGGAATTCTCGGGTGC235090.10397035953603985No Hit
CCCGCCGGGGTCGGCCTGGAATTCTCGGGTGCCAAGG227270.1005119044270525Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG226200.10003868870242126No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)