FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16991032
Sequences flagged as poor quality0
Sequence length37
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG2733951.6090547060355134No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA2309181.3590581196009754No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1216500.7159659283791591No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA1164990.6856499358014275No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1151120.6774868059809434No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG1103840.6496603620074402No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1098910.6467588313646869No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG1045700.6154423109791095No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA972910.5726020644302241No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG961960.5661574882561577No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT894610.5265189306923793No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT800500.47113088834156747No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG797010.46907686360663675No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC769260.4527447185079752No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG650320.38274308470491963No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA604900.35601133586235373No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG579740.341203524306234No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA548740.32295860545727884No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA534540.3146012555329188No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT520230.3061791655739333No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGT519290.3056259325507715No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG507780.29885177074588526No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA495530.29164208507170136No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG481330.28328473514734126No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT476520.2804538299969066No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG462320.2720964800725465No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT460400.27096647219544995No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG449060.2642923631713483No Hit
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC444780.2617733872786538No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA442460.2604079610938288No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC414360.24386982497590493No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG407290.23970880638680453No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGT406810.23942630441753038No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT401790.2364718046555383No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG390040.22955639186601498No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG389400.22917972257364946No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG383680.22581324077313256No Hit
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG365490.2151075932291811No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG357870.21062287446695407No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTC351090.20663253415095678No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG349590.20574971549697513No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG347910.20476095860451562No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG346070.2036780343889647No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG338060.19896378277670246No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC330590.19456734587987357No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG327230.19258983209495456No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT323640.1904769527830917No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC300210.17668732540789753RNA PCR Primer, Index 1 (100% over 26bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA295620.17398590032671354RNA PCR Primer, Index 1 (100% over 22bp)
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCT284820.1676296060180453No Hit
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC284370.16736476042185078No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA280970.16536370480615892No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG280680.1651930265330558No Hit
CCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT262230.1543343570890809RNA PCR Primer, Index 1 (100% over 30bp)
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA258320.15203314313103525RNA PCR Primer, Index 1 (100% over 28bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC255940.15063240420005095No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG246890.1453060649876947No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG242150.1425163580411125No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA239790.14112739002551464RNA PCR Primer, Index 1 (100% over 23bp)
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGA239070.1407036370716034No Hit
CTGGATGATGATAAGCAAATGCTGACTTGGAATTCTC238790.1405388442561935No Hit
CCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA238540.14039170781386323No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT234470.1379963265327262No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC230440.13562448708236205RNA PCR Primer, Index 10 (100% over 37bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA228320.13437677005140122No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG226600.1333644713281689No Hit
ACGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAA226240.13315259485121328No Hit
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTC224700.13224623436645874RNA PCR Primer, Index 1 (100% over 26bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACTGGAA224080.13188133598947963No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT223100.13130456113554492No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG222200.1307748699431559No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT221190.13018043871614154RNA PCR Primer, Index 1 (100% over 25bp)
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG219500.12918579636598884No Hit
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC214380.12617244202706462No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGG214310.12613124382321214No Hit
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGT210950.12415373003829315No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG209400.1232414840958454No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGA207420.12207616347258954No Hit
AGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCT206540.12155824319558695No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGGAATTCT204280.12022812975692118No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT200780.11816821956429721No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC196070.11539616899079468RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT195310.11494887420611061No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC193920.11413079558675422No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGTGG189530.11154707965943447No Hit
CCCGGTCAGCCCCTCTCCGGCCCCGTGGAATTCTCGG188840.11114098307860287No Hit
TACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGC188840.11114098307860287No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA188550.11097030480549976No Hit
CCCGCCGGGCATGGAATTCTCGGGTGCCAAGGAACTC187330.11025227896692798RNA PCR Primer, Index 1 (100% over 26bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA185210.10900456193596716No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGTGGAATT183960.10826887972431574No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT183740.1081393996550651No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT183330.10789809588964343No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG182660.10750377022419827No Hit
TCTGGACGCGAGCCGGGCCCTTGGAATTCTCGGGTGC182490.10740371744341368No Hit
CGACGGGCGCTGGAATTCTCGGGTGCCAAGGAACTCC182060.1071506427626056RNA PCR Primer, Index 1 (100% over 27bp)
CTTCGTGATCGATGTGGTGACGTCGTGCTTGGAATTC181520.10683282804717217No Hit
CGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAG177660.1045610413775926No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG176700.1039960374390443No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC174180.10251290210035505RNA PCR Primer, Index 1 (100% over 27bp)
CTTCGTGATCGATGTGGTGACGTGGAATTCTCGGGTG173960.10238342203110441No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTC173870.1023304529118655No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGGAATTCTC170740.10048830465389036No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)