FastQCFastQC Report
Sat 1 Apr 2023
EGAF00004170917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30035037
Sequences flagged as poor quality0
Sequence length37
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG5628341.8739247765867577No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG5428911.8075256574513292No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG5048401.6808369505254814No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG5008461.6675391476960726No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG3655471.217068585598879No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG3614601.2034611443961265No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA3218001.0714153606669437No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT2606380.8677798532427311No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG2590540.862506012561263No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG2312470.7699241389314753No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT2196090.7311760594801332No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG2004910.6675237323663028No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1996240.6646371036599689No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT1834300.6107200733596565No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC1806280.6013909688208475No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT1746180.5813810051241155No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG1731840.5766065811738471No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT1670920.5563236029973927No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG1606960.5350284735790404No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT1605390.5345057507337181No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA1492600.49695294199238044No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG1218130.4055696685174717No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG1180680.3931008974618543No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA1173930.3908535221714559No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA1154500.3843844107799834No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA1151460.38337225953808546No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA1122110.373600338830946RNA PCR Primer, Index 1 (100% over 22bp)
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA1091440.36338893140035083No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG1051000.3499246563272088No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC1034960.3445842267482474No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG1023690.3408319423745008No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG963830.32090188535476083No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG959460.3194469179445326No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA930850.30992137615811827No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG927930.3089491782547163No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT866050.2883465733702942No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT865740.2882433605791796No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG821980.273673709807649No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG818170.2724051913104019No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGT812780.2706106205229579No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT801570.2668783128184593No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC766410.2551719846391399No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG744840.24799037204448923No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA741170.24676846577548747No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA724720.24129152895666484RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTG723770.24097523169357174No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG723240.24079877111521453No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG716640.23860133749793616No Hit
CCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT709750.2363073499792925RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG699100.23276149118777512No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG685250.22815021003636518No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT681340.2268483970903715No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTTGCT673770.22432800732025068No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA646640.21529522337528664No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC643380.21420982434614613No Hit
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTG629960.20974170932434674No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG614820.20470092978410515No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT612910.2040650058130443No Hit
GTTCGTTGATGAGGCTCAGAGTGAGCGCTGGGTACAG608200.2024968372770774No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG608140.20247686060782943No Hit
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTG590170.19649384816805784No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC589880.19639729426669258No Hit
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTG587470.19559489805189853No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTG584230.19451615791250731No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG577420.19224880595286098No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGAT576260.19186259034739994No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGT569860.18973174562761486No Hit
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCT564880.18807368208003206No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGT553430.18426146769854154No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTC537050.17880783699384156No Hit
TCGAGAGGGGCTGTGCTCGCAAGGTTTCTTTTGGAAT528460.175947843846505No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGA523940.17444293476315675No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG510670.17002476141447737No Hit
ATTCGTGATGACTGATCATTTCTTCACTTTGACCAGA506550.1686530301261157No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC501850.16708819103502354No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG499750.16638900761134406No Hit
GCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGAT495280.16490074575236913No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC481890.16044261906519375RNA PCR Primer, Index 1 (100% over 27bp)
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGT481120.1601862518098446No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT479500.15964688174014902No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA472260.15723636365089214RNA PCR Primer, Index 1 (100% over 37bp)
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTG471680.15704325584816162No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG471240.15689676027367638No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG470020.15649056799896735No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT469730.1563940140976021RNA PCR Primer, Index 1 (100% over 35bp)
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATG466290.1552486850607176No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGA451460.15031111831159055No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA440030.14650556281984936No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTA431390.1436289224481395No Hit
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCG421480.14032944257734725No Hit
ACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGT413360.1376259333391199No Hit
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGA405580.13503562522663115No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC403820.13444964292869024No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT400700.13341085612779502No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCA394470.13133661197087923No Hit
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGT394230.13125670529388728No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTA388740.12942884005769661No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT384450.1280005082064657No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC381370.12697503918506908No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG374460.12467439277667612No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGA373910.12449127330856959No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGC371110.1235590287436636No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC365260.12161130349198504RNA PCR Primer, Index 1 (100% over 31bp)
TGATGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG365120.12156469126373974No Hit
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTG362560.1207123533758257No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTC358450.11934395153233872No Hit
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC358010.1191974559578535No Hit
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGT355560.11838174196356074No Hit
TGATGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG352250.1172796957100469No Hit
AGAAATGAAGAAACTAAAATTGGTCTTAGTATTGAAG347390.1156615855009601No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGT332300.1106374531850918No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC326650.10875631683090652RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT324070.10789732005324316No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG322320.10731466720017692No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC320490.10670537878811337No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC320470.10669871989836402No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCT319380.10633581040702562No Hit
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCT314870.10483423076855208No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG310830.10348913503918775No Hit
TGAGGTAGTAGGTTGTATAGTTTTGGAATTCTCGGGT308820.10281991661938022No Hit
AAGCTATGATGAATTTGATTGCATTGATCGTCTGACA308100.1025801965884044No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG307220.10228720543943395No Hit
TCGAGGAGCTCACAGTCTAGTATGGAATTCTCGGGTG305770.10180443593260763No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTG301860.10050262298661394No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGT301790.10047931687249129No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)