FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004170918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30936562
Sequences flagged as poor quality0
Sequence length37
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC5056151.6343606636057364No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT4863051.5719426095246136No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG4860821.5712217795888246No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG4444631.4366916401376468No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGT3134121.0130796046438515No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT2527850.8171076023250419No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA2515690.8131769781011865No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG2470120.7984468345254394No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG2459360.7949687492747255No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG2222860.7185219870262248No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG2194400.709322516186511No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT2146400.6938068942502402No Hit
CCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTG1684020.5443462011066388No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTC1672960.540771143218823No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG1623960.5249322791588801No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC1605070.5188262354427101No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC1493950.4829075706602434No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCT1421240.45940463584802993No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG1406200.45454307430799845No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGT1347290.4355008808024628No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGC1332330.4306651786323251No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA1327310.4290425031714901No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA1294820.41854036657337684No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG1263300.40835177483522567No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT1257380.40643818146308563No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG1247820.4033479867607784No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA1229220.3973356832604735No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATT1225650.3961817088789633No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC1157620.37419154720553627No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT1151230.3721260300352702No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG1125680.3638671937754428No Hit
GGAGGTGATGAACTGTCTGAGCCTGACCTTGTAGAAT1125390.36377345355957785No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT1123170.36305585604502527No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA1041390.336621115171104No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1004600.32472903744119985No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA988590.31955393104120616No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGA958850.30994071028319176No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTG912640.2950036917482945No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG903960.29219795011481886No Hit
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGC895060.2893210952141353No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG881660.2849896507569264No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA877440.2836255689950292No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG869140.28094265936854906No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT832460.26908613827224886No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG825410.26680728130035913No Hit
CTCCATGATGAACACAAAATGACAAGCATATGGCTGA824520.2665195958102907No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGA819390.2648613637158518No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCT815290.26353607100879534No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGA814060.26313848319667843No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG803110.25959898194246667No Hit
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC773020.24987262644116692No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA762590.24650121109126474No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA752080.2431039363714688RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTG746410.24127115353024683No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT731730.23652595915473737No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG720220.23280544231126912No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG718690.23231088186205046No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT717740.2320038018445618No Hit
GTTCGTTGATGAGGCTCAGAGTGAGCGCTGGGTACAG715200.2311827668504341No Hit
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCT714850.23106963210714881No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA710240.2295794859170195No Hit
GGTCAATGATGTGTTGGCATGTATTATCTGAATCTAT682550.2206289115125333No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGA679840.21975292535738136No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGAT671250.21697627551503623No Hit
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTC656770.21229572956426118No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA651090.21045971430180252No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGT639870.2068329376741992No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC628600.20318999894041231No Hit
TACCCTGTAGATCCGAATTTGTGGAATTCTCGGGTGC626680.20256937406296147No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATG620010.20041334909806718No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTG617630.19964403284372712No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG614220.19854177720200453No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC595590.19251977643798945No Hit
CCCCCCACTGCTAAATTTGACTGGCTATGGAATTCTC577640.18671758031807156No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT564600.18250250302538465No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTC560910.18130973958903385No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT553600.17894683966498928No Hit
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCG547750.1770558732415063No Hit
CCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGG547260.17689748460090685No Hit
GGAGCCATTGTGGCTCAGGCCGGTTGCGCCTGCCCTC546610.17668737722051983No Hit
TTCCCTTTGTCATCCTTCGCCTTGGAATTCTCGGGTG545320.17627039488098256No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA541330.17498065880753008No Hit
GCTCCGTGAGGATAAATAACTCTGAGGTGGAATTCTC539440.1743697311937894No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT524780.16963100166075337No Hit
GGTCAATGATGAGTTGGCATGTATTCTGAATCTAAAG494660.1598949488957435No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC494310.15978181415245818No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC487750.15766134582116784No Hit
AATTGCACGGTATCCATCTGTTGGAATTCTCGGGTGC485770.15702132641629668No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC478010.15451296753659957No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGAT460040.14870430657420822No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG454150.14680041046577832No Hit
TGGAGGTGATGAACTGTCTGAGCCTGACCTTGTAGAA452710.1463349418076902No Hit
TGTCGCTGATGCCATCACCGCAGCGCTCTGACCTGGA447160.1445409480213089No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG437870.14153802869239315No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATGGAATT436800.1411921596200638No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGG419880.13572290288752836No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG404820.13085487650502342No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT402510.1301081871993404RNA PCR Primer, Index 2 (100% over 37bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC396810.1282657070944082RNA PCR Primer, Index 1 (100% over 31bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG393430.12717314871639585No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT392890.12699859796961277No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCG387950.12540178187867157No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCG380440.12297423352989256No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGC379980.1228255421530033No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG379650.12271887225219144No Hit
GTACATGATGACAACTGGCTCCCTCTACTGAACTGCC368590.11914381436437572No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG363600.11753083616725091No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA363120.1173756799478882No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT361660.11690374644732662No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA360100.11639948873439783No Hit
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTG350950.11344182330279623No Hit
GGTCAATGATGTAATGGCATGTATTAGCTGAATCTAA349800.11307009486057308No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTG347280.11225552470891884No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA344220.1112664038104816No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGA337230.10900694136601217No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG334110.10799842594015456No Hit
TGGCCAAGGATGAGAACTCTAATCTGATTTGGAATTC327420.10583593613278683No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAAT326030.10538662958088232No Hit
CCCCCCACTGCTAAATTTGACTGGCTTGGAATTCTCG323690.10463024301148913No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGAT321610.10395789939425072No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGT319210.10318211829743718No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT319180.10317242103372701No Hit
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGT315010.1018245013780135No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTC313630.10137842724734572No Hit
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGA313420.10131054640137452No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG312880.10113599565459148No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)