FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12794841
Sequences flagged as poor quality0
Sequence length37
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG3176872.4829304248485777No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG2555761.9974925831434718No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG2203331.7220456276088152No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT2082291.62744499912113No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG2039891.5943066428101764No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG1993981.5584249933234808No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG1788081.3975007583134484No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT1629721.2737321237520653No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1522161.1896669915632403No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT1499121.1716597337942691No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG1303301.0186136740581613No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA1262500.9867258217589417No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1186130.9270377021488583No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC1125420.8795888905536224No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA1010670.7899043059620671No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG1009760.7891930818053932No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG960670.7508260555953763No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT948420.7412518842555371No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG907660.7093952945566108No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA906900.708801305151037No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG860880.6728336835135349No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT851090.6651821620917369No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG813520.6358187647662054No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC783280.6121842389444309No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA745000.5822659304636923No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG696870.5446492066607158No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG674820.5274156982490052No Hit
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTG642910.5024759588649831No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG626050.489298772841335No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG622550.48656329531566667No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC591390.46220972968714497No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT585010.45722334494035527No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC541910.4235378931242678No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT532450.41614428815488996No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGT501960.39231437108128187No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG489520.38259170239004925No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG484410.37859790520257347No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA428050.3345489013892396No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA422400.33013305909780355No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA412040.3220360456218252No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT404160.31587731336403474No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAA402960.31493943535523417No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT392750.30695965663035596No Hit
TGAGAACTGAATTCCATGGGTTGTGGAATTCTCGGGT387970.3032237758953003No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG386620.30216866313539964No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA348810.2726176902081081No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTC326900.25549360089742423No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGT326710.2553451035460308No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG321300.25111683685635483No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA320370.2503899813995344No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG315430.24652905026330535No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC311960.24381701968785702No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT311950.24380920403778367No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT310350.24255870002604954No Hit
TTCCCTTTGTCATCCTTCGCCTTGGAATTCTCGGGTG309000.2415035872661489No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA308530.24113625171270203No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT303270.23702521977412616No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTG283390.22148770742832993No Hit
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTG281420.21994802436388228No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG276810.2163450096800734No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG276740.21629030012956002No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG275770.21553218207244623No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC270940.2117572230870239RNA PCR Primer, Index 1 (100% over 27bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGT270490.2114055188337237No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG260120.203300689707672RNA PCR Primer, Index 3 (100% over 37bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG255490.19968204372371648No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG253000.19773594685545526No Hit
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTG246740.19284334990954557No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG242930.18986558723160374No Hit
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGT230770.18036175674242455No Hit
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTG229100.17905654318017708No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG229060.1790252805798837No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGAT228600.17866576067651016No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG224070.175125271193288No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGC218980.17114710530595886No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGC214900.1679583200760369No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGC212170.1658246476060156No Hit
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTG211760.16550420595300872No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCT207920.16250299632484685No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCA204470.1598065970495452No Hit
TCGAGGAGCTCACAGTCTAGTATGGAATTCTCGGGTG204120.15953304929697837No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGA198850.15541420170832917No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTG198150.1548671062031955No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG197000.15396830644476162No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG196900.15389014994402822No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGT192400.15037310741102605No Hit
ATAGGTTTGGTCCTGGCCTTTCTATTAGCTCTTAGTA188710.14748913253396426No Hit
AGCTACATCTGGCTACTGGGTCTTGGAATTCTCGGGT188580.1473875290830109No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA188020.14694985267890392No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA186040.14540235396438297No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGT185340.14485525845924932No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT184590.14426908470374897No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGC182080.14230735653534107No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC181140.1415726854284473No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT179480.14027528751627316No Hit
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCC177080.138399531498672No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA176850.13821977154698523No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC176560.13799311769485842No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG172550.13485904201544982No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT171430.13398368920723594No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG171030.13367106320430244No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG167750.1311075299802475No Hit
GATCGATGATGACTTCCATATATACATTCCTTGGAAA167040.13055261882504052No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA166730.130310333672767No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG165240.12914580181183963No Hit
CTAGACTGAAGCTCCTTGAGGTGGAATTCTCGGGTGC163360.12767645959805207No Hit
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGT161980.1265978998879314No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG161610.12630872083521788No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGA161520.12623837998455784No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGA161300.1260664356829444No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTG161000.12583196618074424No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGGAATTCTC159090.12433917701673666No Hit
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGC158420.12381552846182302No Hit
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC156870.12260410270045559No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTC156430.12226021409722872No Hit
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCT155560.1215802525408483No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG153850.12024377637830748No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG147000.11489005607807083No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGA146730.11467903352609071No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG145910.11403815022007699No Hit
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGA142980.1117481647485889No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCAC142660.11149806394624208No Hit
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGT139370.10892671507211384No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC139180.1087782177207204No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGAT138990.10862972036932698No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA135920.10623031579681218No Hit
CAGTGCAATAGTATTGTCAAAGCTGGAATTCTCGGGT134120.10482349878361129No Hit
CCCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA133320.10419824677774425RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGCTCTGG132960.10391688337510407No Hit
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTG132190.10331507831945705No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAC129780.10143150665178254No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC129430.1011579588992157No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGT129330.10107980239848233No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAAT129110.10090785809686888No Hit
GTTCTTGTAGTTGAAATACAACGATGGTTTTTCATAT128810.10067338859466875No Hit
CCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTG128170.1001731869899751No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCG128010.10004813658880168No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)