FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19403986
Sequences flagged as poor quality0
Sequence length37
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA3330381.7163380761045695No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA1991811.0264952778259067No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT1852640.9547729007844058No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1559440.8036699263749212No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1476960.7611631960567278No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT1473820.7595449718423833No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT1421520.732591746870978No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1327960.6843748495798749No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC1305050.6725679971115214No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1253360.645929140538444No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG1210310.6237429773449641No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT1174430.6052519312269139No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1103530.568713046896653No Hit
GTTCGTTGATGAGGCTCAGAGTGAGCGCTGGGTACAG994670.5126111717458465No Hit
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTC980480.5052982412994937RNA PCR Primer, Index 1 (100% over 26bp)
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC955000.49216691869392193No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT941650.485286888992808No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG924550.4764742666790215No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG918010.47310382516252075No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA870290.448510939968726No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGT866040.44632066834103057No Hit
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCT862460.4444756865934659No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG844290.4351116311875302No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT821970.42360883995690374No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG783870.40397369901215135No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC777270.4005723360138479No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT757740.3905073936870497No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTA753280.38820889687304455No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTA730060.37624228341537663No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGT692490.3568802822265487No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG682890.3519328451381072No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA664380.34239356800195586No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG662850.3416050702159855No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG615420.31716163885090415No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA615060.31697610996008757No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC609270.3139921869661213No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG602840.31067843483292557No Hit
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCG559780.2884871180591452No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT559500.2883428178107323No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG525110.2706196551574506No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA522360.2692024205748242No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG507380.26148235728473523No Hit
GGTCAATGATGTGTTGGCATGTATTATCTGAATCTAT506080.2608123918456754No Hit
GGTCAGTGATGAGCAACATTCACCATCTTTCGTTTGA489850.252448131018029No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTC488740.25187608360467795No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG483760.24930960061504887No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA476570.2456041763790182No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATG470430.2424398780745358No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG464930.23960540890928284No Hit
AGTCTGTGATGAATTGCTTTGACTTCTGACACCTCGT460920.23753882320879843No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG459250.23667817529862165No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTG450340.23208633525091185No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC447040.23038565375176007RNA PCR Primer, Index 1 (100% over 27bp)
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGA441800.2276851776743191No Hit
CGGCCGGTGATGAGAACTTCTCCCACTCACATTCGAG441710.22763879545161492No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA426410.2197538175919113RNA PCR Primer, Index 1 (100% over 22bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC420870.21689873410545646No Hit
ATTCGTGATGACTGATCATTTCTTCACTTTGACCAGA416770.2147857661822679No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG404820.2086272377232183No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA403260.20782327919634655No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG391520.20177297592360663No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC385740.19879420650994079RNA PCR Primer, Index 4 (100% over 37bp)
TGCCTCTGATGAAGCCTGTGTTGGTAGGGACATCTGA385700.19877359218873894No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGA377630.1946146528862678No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG369250.1902959525944824No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA365880.1885591960332274No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG364980.188095373806186No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGA363460.1873120296005161No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT357630.18430749228534798No Hit
AATACATGATGATCTCAATCCAACTTGAACTCTCTCA356490.18371998413109555No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC350610.1806896789144251No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG349870.18030831397219108No Hit
GTTCTTGTAGTTGAAATACAACGATGGTTTTTCATAT329130.16961978842903722No Hit
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCT322860.16638849358064883No Hit
ATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCC320640.16524439875394675RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAA319890.16485788023141223No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT309800.1596579177082482RNA PCR Primer, Index 1 (100% over 25bp)
CCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTG306990.15820976164381895No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGAT292970.15098444206257414No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCT291220.15008256550999366No Hit
GCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGAT287820.1483303482078373No Hit
GGTCAATGATGAGTTGGCATGTATTCTGAATCTAAAG284320.14652659510267632No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG269570.13892506415949796No Hit
CTTCGTGATCGATGTGGTGACGTGGAATTCTCGGGTG267030.13761605476318114No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT255770.13181312334486328No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT254530.13117407938760622No Hit
TGGAGGTGATGAACTGTCTGAGCCTGACCTTGTAGAA252580.13016913122901655No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG251280.12949916578995677No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGT250610.12915387590982597No Hit
CTGGATGATGATAAGCAAATGCTGACTTGGAATTCTC249360.12850967837226845No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT247620.12761295539998843No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT247210.12740165860766958No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG247090.12733981564406407No Hit
TGCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGA246700.12713882601234613No Hit
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC245030.12627817810216932RNA PCR Primer, Index 1 (100% over 27bp)
GTCGCGTGATGACATTCTCCGGAATCGCTGTACGGCC245010.12626787094156838No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGA244760.12613903143405691No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTG242110.12477333265443502No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC238800.1230674975749828RNA PCR Primer, Index 1 (100% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT235710.12147504126214068No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG234590.12089784026848915No Hit
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGA234370.12078446150187905No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT234160.12067623631556938No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGG231510.11931053753594752No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGC230270.11867149357869047No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG229650.11835197160006197No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA228100.11755316665349068No Hit
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGA225500.1162132357753711No Hit
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGT224740.11582156367253614No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG223920.11539897008789844No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG223910.11539381650759797No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA220640.1137085957493476No Hit
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC220110.1134354559934232No Hit
CCCTGGCGGAGCGTGGAATTCTCGGGTGCCAAGGAAC219870.11331177006621218RNA PCR Primer, Index 1 (100% over 24bp)
AGATTTCAACTTAACTTGACCGCTCTGACCATGGAAT219840.1132963093253108No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC217240.11195637844719122No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG216430.11153893844285397No Hit
GGAGGTGATGAACTGTCTGAGCCTGACCTTGTAGAAT215430.11102358041280797No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCAC214580.11058552608726886No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA206970.10666365147861889No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT206110.10622044357277932No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA204730.10550924949131585No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC203470.1048598983734579No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG203310.10477744108865054No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA198890.10249955859584726RNA PCR Primer, Index 1 (100% over 23bp)
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTC197460.1017625966128815No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG196240.1011338598162254No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC195830.10092256302390652No Hit
AAGCTATGATGAATTTGATTGCATTGATCGTCTGACA194740.1003608227711564No Hit
GGGCGGTGATGACCCCAACATGCCATCTGAGTGTCGG194660.10031959412875271No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)