FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15937737
Sequences flagged as poor quality0
Sequence length37
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG3569942.2399290438786887No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG3337342.0939861160966577No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT2999011.8817037826637497No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG2987811.8746764361841333No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG2527151.5856391657109163No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG2453711.5395598509374324No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1956351.2274954719104727No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG1938731.2164399500380763RNA PCR Primer, Index 5 (100% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1650351.0354983270209566No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG1628121.021550299142218No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT1533810.9623762771339495No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT1404340.8811414067128853No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1376300.8635479428478459No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG1355690.8506163704420521No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA1352900.8488658082386478No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC1170300.7342949629549038No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG1035440.6496781820405243No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG875450.5492937924625058No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT872110.5471981373516203No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG776200.4870202087034063No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA758710.4760462542455055No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG753430.4727333623336864No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG740700.46474603012962257No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG691910.4341331520277942No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT678610.4257881780832498No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA677480.42507916901878856No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG580590.36428634755360817No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTTGCT576140.3614942322112606No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG570270.35781114972596173No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC512690.3216830595209345No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG469120.29434542683192727No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG467830.2935360271034715No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG466790.29288348778750706No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC452890.28416204885298335No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT451810.28348441187102036No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC441370.276933921045378No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG434600.27268614107510997No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG434030.2723284993346295No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA433410.27193948551165076No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC421130.2642345020500715No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG418670.2626909955911558No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG413980.2597482942528164No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG409030.256642458085486No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG408570.2563538349265018No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC402540.25257036177720843RNA PCR Primer, Index 1 (100% over 27bp)
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA399090.2504056880848266No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG395770.24832258180694033No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG391070.24537360605210137No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGT384310.24113210049833297No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG369490.23183341524584075No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG363970.22836993733802985No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA361360.2267323146316193No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC357110.2240656876192649No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT352000.22085946078793994No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG343700.21565169509322435No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTC336820.21133489654146004No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTG334630.2099607993280351No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT332730.2087686601931002No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA332550.20865572069610636RNA PCR Primer, Index 1 (100% over 23bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG324710.203736578160375No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG323670.20308403884441056No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA323260.20282678776792465RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT320700.20122053714401233No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG301220.18899797380267977No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC299800.18810700665972843RNA PCR Primer, Index 1 (100% over 26bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG291840.18311257112600116No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA290100.18202082265506075RNA PCR Primer, Index 1 (100% over 22bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG286970.180056930290668No Hit
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTG283420.17782951243328962No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATG276210.17330565813703666No Hit
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTG275250.17270331415306953No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGT274850.17245233749308325No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT272140.17075197062167607No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT268650.16856219926329566No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT262630.1647850005305019No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT261530.1640948147155396No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC259180.16262032683812014No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAA257250.16140936445368625No Hit
GTTCGTTGATGAGGCTCAGAGTGAGCGCTGGGTACAG254060.15940782559029554No Hit
CCCTGGCGGAGCGTGGAATTCTCGGGTGCCAAGGAAC247560.15532945486551822RNA PCR Primer, Index 1 (100% over 24bp)
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCT247270.15514749678702816No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC243450.15275066968415907No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC242400.15209185595169503No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTA241990.15183460487520908No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA238130.14941268010634132No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTG233800.14669585776198968No Hit
TCGAGGAGCTCACAGTCTAGTATGGAATTCTCGGGTG221900.1392293021273974No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG221360.13889048363641587No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG218780.13727168417950428No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGA213300.13383330393769202No Hit
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTG211680.13281684846474753No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTA210390.13200744873629172No Hit
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTG203910.12794162684451374No Hit
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGT202320.12694399462106823No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTG198970.124842065093683No Hit
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGC195800.12285307506329161No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGC194870.12226955432882347No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGA190800.11971586681346293No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCG190090.11927038324198724No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGT188590.11832922076703864No Hit
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGT186340.11691747705461573No Hit
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGT182280.11437006395575483No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA180050.11297086907633123No Hit
GGTCAATGATGTGTTGGCATGTATTATCTGAATCTAT177430.11132697195342098No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG175560.11015365606798506No Hit
GTTCTTGTAGTTGAAATACAACGATGGTTTTTCATAT175280.10997797240599465No Hit
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTG171800.10779447546411389No Hit
TGAGAACTGAATTCCATGGGTTGTGGAATTCTCGGGT170410.10692233157066151No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTC169370.10626979225469714No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCT169310.10623214575569918No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA169030.1060564620937088No Hit
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGT168210.10554195994073687No Hit
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGA168070.10545411810974167No Hit
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTG167960.10538509952824544No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT166990.10477648112777868RNA PCR Primer, Index 1 (100% over 25bp)
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC164550.1032455235018623RNA PCR Primer, Index 1 (100% over 27bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGT163200.10239847727440853No Hit
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGC162920.10222279361241814No Hit
TTCTGTGATGAAAATGTTTCTGAAGTGGAATTCTCGG162270.10181495653994041No Hit
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGT162200.10177103562444278No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGT161730.10147613804895889No Hit
TGATGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG161600.10139457063446335No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTG160710.10083614756599385No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGA159880.10031537099652228No Hit
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGG159740.10022752916552709No Hit
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCG159670.10018360825002946No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)