FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15557786
Sequences flagged as poor quality0
Sequence length37
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG3649972.3460728923768457No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG2683091.7245962889578248No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT2535081.629460644335897No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG2339221.503568695442912No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGT2230291.4335523062214637No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG2125391.3661262598675672No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG2059561.3238130412643547No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT1903001.223181756067348No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG1876481.206135628809909No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1766141.1352129409673073No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1383990.8895803040355486No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG1210090.7778034740932932No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1174090.7546639348298017No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT1106620.7112965816601411No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1059870.6812473188665791No Hit
CCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTG987470.6347111343477793No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG979990.6299032523008093No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA948340.6095597406983231No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA912540.5865487544307397No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG899340.5780642567007928No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG888570.5711416778711316No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT866160.556737314679608No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG846500.5441005551818234No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT701490.45089320549852013No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG650810.41831787633536033No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG649930.4177522431533638No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGT594630.38220733978472254No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA582970.37471270012326946No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG576190.37035475356197856No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT573650.3687221305139433No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG558570.35902923462245845No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT555590.357113794983425No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG551990.35479984105707585No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG515740.3314996105486989No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA493790.31739091924776447No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA454700.2922652361974898No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT449830.289134970747123No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA438140.2816210481362837RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT430020.2764017965024072No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA427470.27476274580457655No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG424110.2726030554733173No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG407440.26188816326436165No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC392910.2525487881116246No Hit
CCCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA389580.25040838072975163RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG389090.25009342588977634No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG376780.24218098899162133No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGT369550.23753379818953674No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA359250.23091331890025996No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG349340.22454351795300437No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA333400.2142978441791139No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT325010.2089050460007613No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG322130.20705388285968193No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT301970.19409574087212667RNA PCR Primer, Index 1 (100% over 25bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC291780.18754596573059945No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG283880.18246812239222213No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC283280.18208246340449727No Hit
GTTCGTTGATGAGGCTCAGAGTGAGCGCTGGGTACAG281390.18086763759316396No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT277480.17835442652315694No Hit
AGCTACATCTGGCTACTGGGTCTTGGAATTCTCGGGT277010.17805232698277249No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG273630.17587978135192242No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG270200.17367509747209534No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT262820.16893149192307955No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA259470.16677822924161576No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG256510.1648756449021731No Hit
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCC251680.161771090050988No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG250590.1610704762232878No Hit
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTG247550.15911647068548185No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATG245680.15791450017373937No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC244640.1572460245950163No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGC243370.15642971307099865No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC242830.15608261998204628No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG237280.1525152743455913No Hit
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGT236670.15212318770807107No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGT234930.15100477664366896No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGT234670.1508376577489882No Hit
GTACATGATGACAACTGGCTCCCTCTACTGAACTGCC231620.14887722456138683No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGT228880.1471160485174433No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGA223760.14382509182219116No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTG222420.14296378674960564No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA222280.14287379965246982RNA PCR Primer, Index 6 (100% over 37bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG210980.13561055538365163No Hit
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCT209430.1346142696653624No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGA208350.13392008348745765No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT205450.1320560650467875No Hit
CCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT204120.13120118762399738RNA PCR Primer, Index 1 (100% over 30bp)
CCCCGGGGAGCCCGGCGGGCTGGAATTCTCGGGTGCC201970.12981924291798333No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC199700.12836016641442427RNA PCR Primer, Index 5 (97% over 35bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA199300.1281030604226077No Hit
GGAGGTGATGAACTGTCTGAGCCTGACCTTGTAGAAT196080.12603335718848427No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC194390.12494708437305925No Hit
AGAAATGAAGAAACTAAAATTGGTCTTAGTATTGAAG193260.12422075994617743No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG190600.12251100510059722No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGT189970.12210606316348611No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT189590.12186181247126036No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT187210.12033203181995177No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTG187060.12023561707302055No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTA185770.11940645024941209No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG184980.11889866591557437No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG184040.11829446683480542No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTG182830.11751672120956028No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGAT172680.11099265666721474No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC168370.10822233960539114RNA PCR Primer, Index 1 (100% over 24bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC166290.10688538844794498RNA PCR Primer, Index 1 (100% over 31bp)
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG164100.10547773314274922No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTA163200.10489924466116193No Hit
ATTCGTGATGACTGATCATTTCTTCACTTTGACCAGA163140.10486067876238946No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGT162830.10466142161873161No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC161920.10407650548734891No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG161870.10404436723837185No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG159860.10275240962949354No Hit
ACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGT159680.10263671193317608No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC159420.10246959303849533RNA PCR Primer, Index 1 (100% over 27bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA157770.10140903082225196No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG155650.10004636906562413No Hit
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGT155580.10000137551705622No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)