FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17735672
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG3566502.0109190111319153No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT2049681.155682175448441No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA2047541.1544755676582201No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1708710.9634312136579882No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1644420.9271822347639267No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT1586810.894699676448685No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC1513230.8532126665400668No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT1257340.7089328219421288RNA PCR Primer, Index 1 (100% over 25bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTC1230690.6939066081059686No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT1203700.6786886902283714No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1168800.6590108342102854No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA1162130.6552500519856254No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC1039000.585824997214653No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC1026860.5789800352645222RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG979170.5520907242759112No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG964850.5440166011189201No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC956830.5394946410826723RNA PCR Primer, Index 1 (100% over 24bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG923210.5205384943970547No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG902620.5089291231818No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA902170.508675397244604No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG848150.47821700807276996No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG755330.425881804760485No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTC724460.4084762054688427No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATG704140.3970190698159055No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG695560.39218136194670267No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC665350.3751478940296144No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT633230.3570375004679834No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTC582690.32854125854379806No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG577040.32535558844344886No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA571890.32245183605109523No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG566400.31935637961730456No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG560230.3158775151006401No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG556230.3136221734366761No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGT537400.3030051525535655No Hit
CCCTGGCGGAGCGTGGAATTCTCGGGTGCCAAGGAAC529570.2985903212463559RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGGCGGATGGAATTCTCGGGTGCCAAGGAACTCC509830.28746021013469353RNA PCR Primer, Index 1 (100% over 27bp)
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGA500000.281917707995502No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA484500.27317825904764137RNA PCR Primer, Index 1 (100% over 22bp)
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC472180.2662318067226322No Hit
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTC466640.26310815851804203RNA PCR Primer, Index 1 (100% over 26bp)
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG460180.25946578173074014No Hit
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCT455800.25699618260869955No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG455540.2568495854005419No Hit
GTTCGTTGATGAGGCTCAGAGTGAGCGCTGGGTACAG451890.25479158613217473No Hit
GGTCAATGATGTGTTGGCATGTATTATCTGAATCTAT446060.2515044256569472No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC440190.24819471176508No Hit
TGTCGCTGATGCCATCACCGCAGCGCTCTGACCTGGA440160.2481777967026003No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG432030.24359381477059341No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA424290.23922972865082306No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGA423930.2390267479010663No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTG423800.23895344929698745No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC421770.23780886340252572No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGAT413160.23295424047084318No Hit
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCG407090.22953175949577778No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA405510.228640899538512No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA387060.21823813611347798No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG382550.21569523838735855No Hit
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTC369940.208585273791712RNA PCR Primer, Index 1 (100% over 26bp)
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT363840.20514587775416684No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGA363550.20498236548352947No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTA355700.2005562574680001No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT352770.19890421969914643No Hit
CGGCCGGGCGCGTGGAATTCTCGGGTGCCAAGGAACT346220.19521109772440537RNA PCR Primer, Index 1 (100% over 25bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT343990.19395374474674543No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA342920.19335044085163505RNA PCR Primer, Index 7 (100% over 37bp)
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC342320.19301213960204044No Hit
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC333470.18802219617052007RNA PCR Primer, Index 1 (100% over 27bp)
GCCCTCGGCCTGGAATTCTCGGGTGCCAAGGAACTCC328440.1851861040280853RNA PCR Primer, Index 1 (100% over 27bp)
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTA326120.1838780058629862No Hit
CCCGCCGGGCATGGAATTCTCGGGTGCCAAGGAACTC310590.1751216418526459RNA PCR Primer, Index 1 (100% over 26bp)
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTC300100.16920700833890026No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTC296810.16735198982028987No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC295000.16633144771734615No Hit
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC294400.16599314646775157No Hit
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCC290200.16362503772058934No Hit
ATTCGTGATGACTGATCATTTCTTCACTTTGACCAGA283120.15963308297537304No Hit
AGTCTGTGATGAATTGCTTTGACTTCTGACACCTCGT282210.1591199927468212No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA279200.15742284814468827No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG272980.15391579185722423No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG272280.15352110706603053No Hit
CCCCGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC271780.15323918935803504RNA PCR Primer, Index 1 (100% over 27bp)
ACGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAA268500.15138980919358455No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT265490.14969266459145164No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTG260880.1470933833237331No Hit
GCTCCGTGAGGATAAATAACTCTGAGGTGGAATTCTC258020.14548081403399882No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGC256140.14442080345193575No Hit
GGTCAATGATGAGTTGGCATGTATTCTGAATCTAAAG250210.1410772594351091No Hit
TTCCCTTTGTCATCCTTCGCCTTGGAATTCTCGGGTG249860.14087991703951225No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG249750.14081789514375323No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT248580.14015820770704374No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG247760.13969586266593112No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGC246390.13892340814602344No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG243050.1370401978566135No Hit
GCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC237570.1339503797769828RNA PCR Primer, Index 1 (100% over 27bp)
CCGCCGGGCATGGAATTCTCGGGTGCCAAGGAACTCC229280.12927618417841738RNA PCR Primer, Index 1 (100% over 27bp)
GCCCCGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAG229190.1292254389909782No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTC228160.12864468851250746No Hit
AAACCTGATGTTACATTGTAGTGTGCTGATGTGGAAT227460.12825000372131376No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT225570.12718435478509077No Hit
CGACGGGCGCTGGAATTCTCGGGTGCCAAGGAACTCC224700.12669381797317858RNA PCR Primer, Index 1 (100% over 27bp)
CTGGATGATGATAAGCAAATGCTGACTTGGAATTCTC224070.12633860166110425No Hit
CGGCTCCGGGACTGGAATTCTCGGGTGCCAAGGAACT223570.12605668395310873RNA PCR Primer, Index 1 (100% over 25bp)
ACCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTC223180.12583678814087226RNA PCR Primer, Index 1 (100% over 26bp)
CGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAG221160.12469784060057043No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA220740.1244610297258542No Hit
CCCGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCA219290.12364346837266725RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGAT217430.12259473449892397No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG217230.12248196741572578No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG214070.12070024750119421No Hit
GCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG213220.12022098739760186RNA PCR Primer, Index 1 (100% over 29bp)
TGCCTCTGATGAAGCCTGTGTTGGTAGGGACATCTGA211480.1192399137737775No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA211190.11907640150314013No Hit
CCCGGTCAGCCCCTCTCCGGCCCCGTGGAATTCTCGG210050.11843362912891038No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCT209600.11817990319171441No Hit
CCCGGCGGGCGTGGAATTCTCGGGTGCCAAGGAACTC208040.11730031994276846RNA PCR Primer, Index 1 (100% over 26bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA207080.11675903794341709RNA PCR Primer, Index 7 (100% over 35bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG207070.11675339958925719No Hit
CCCCCCTCGCGGCCTGGAATTCTCGGGTGCCAAGGAA206060.11618392581910628RNA PCR Primer, Index 1 (100% over 23bp)
GCCGGGCGCGTGGAATTCTCGGGTGCCAAGGAACTCC200470.11303208584371655RNA PCR Primer, Index 1 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC198360.11184239311597553No Hit
GGCGCGACCGTGGAATTCTCGGGTGCCAAGGAACTCC197950.11161122059541921RNA PCR Primer, Index 1 (100% over 27bp)
AATACATGATGATCTCAATCCAACTTGAACTCTCTCA197520.11136877136654309No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT197210.11119398238758588No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG192570.10857778605738763No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC192010.10826203822443266RNA PCR Primer, Index 1 (100% over 31bp)
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGA190990.10768692610012183No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCAC189000.10656489362229973No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA188720.10640701970582225RNA PCR Primer, Index 1 (100% over 23bp)
AGATTTCAACTTAACTTGACCGCTCTGACCATGGAAT188310.10617584718526595No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG185840.10478317370776816No Hit
TTTTATGAGTGAAACATAAGAGTCTGACATGGAATTC184010.10375135489650462No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA182280.1027759196268402No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG181130.10212750889845054No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTG179690.10131558589942348No Hit
CCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG178930.10088707098327032RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)