FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15629877
Sequences flagged as poor quality0
Sequence length37
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG5045243.2279460676497966No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT2306051.4754114827647076No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG2073041.3263316147657462No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1858501.1890688583153917No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG1573161.0065082405958792No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT1571941.0057276842293768No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG1412280.9035771682656235No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1397020.893813815681339No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT1303820.8341844276829562No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG1285060.8221817740472303No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT1231630.787997243996226No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC1211050.7748301538137504No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG1169990.7485599534788405No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG1157330.7404600816756267No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA1118260.7154630839385364No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1007560.6446371906829464No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA951470.6087507918328467No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA926200.5925830382414398No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG911370.5830947997863323No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG828030.52977384275001No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC785370.5024799619344413No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA762770.48802047514513386No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT735950.4708610310880885No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG729710.46686867721351877No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG700780.4483592545226044No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG700530.4481993044475014No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG688780.44068165091766237No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC671400.4295619216965047No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG608950.38960639293578575No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG590490.3777956793901833No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA578340.3700221057401795RNA PCR Primer, Index 1 (100% over 22bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG531020.33974675552469163No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG504010.322465749410568No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT498530.31895964376431113No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG469480.30037344503734736No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG466130.2982301140309677No Hit
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGT451420.28881865161190967No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG450380.2881532592994814No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT424640.2716847995668808No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC416670.26658559117259845No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG404600.2588632015466277No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG378270.24201725963678408No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT378150.24194048360073467No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG365800.23403894989064852No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG361430.23124302257784882No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC355020.227141902652209No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT347360.22224103235105433No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG336930.21556791521775892No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATG320870.2052927223931449No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG318890.20402591779832943No Hit
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCC317030.20283588923956344No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA309010.19770469083026054No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG301010.19258628842696587No Hit
TCGAGGAGCTCACAGTCTAGTATGGAATTCTCGGGTG297820.1905453254686521No Hit
AGCTACATCTGGCTACTGGGTCTTGGAATTCTCGGGT295750.18922093884679964No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA287020.1836354822242043No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC286230.18313003998687896RNA PCR Primer, Index 1 (100% over 31bp)
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTG286110.18305326395082958No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG285870.18289971187873072No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTG280360.1793744122234615No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA276050.1766168729286865RNA PCR Primer, Index 1 (100% over 23bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG275360.17617541072140236No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA260220.1664888341731672No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA258770.16556112373757004No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTT257690.16487013941312526RNA PCR Primer, Index 8 (100% over 37bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT256630.1641919510946887No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCA249780.15980931903686765No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGT248480.15897757864633227No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA247460.1583249823399122No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC243900.15604729327044609No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC243180.15558663705414957RNA PCR Primer, Index 5 (100% over 35bp)
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTC241470.15449257854044532No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGT237330.15184380529674035No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA236140.15108244293925024No Hit
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTG235670.15078173679805668No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG228140.1459640405359556No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT224230.14346242136134532No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG219520.14044896194640558No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT218570.13984115166101435No Hit
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG213050.13630945400274103No Hit
ACGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAA211990.1356312656843045No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGAT211510.1353241615401068No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG211330.13520899748603266No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA210500.13467796323669087No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT207630.13284173637450888RNA PCR Primer, Index 1 (100% over 25bp)
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG205380.13140218569858225No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA204680.130954325488294No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA204440.13080077341619514No Hit
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGG202600.12962354086343736No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC201870.12915648664413676RNA PCR Primer, Index 1 (100% over 26bp)
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC198630.12708353367080238No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGT193830.1240124922288256No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATTGGAA193240.12363501005158262No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT192260.12300800575717902No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA191490.12251535952586191No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC190060.12160044509627299No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTA189940.12152366906022358No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGAT189050.12095424679285703No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTG189010.12092865478084056No Hit
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTG186620.11939953206285628No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC185840.11890048782853505RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGCCGCCGGTGAAATACCACTACTCTGATGGAAT183270.11725620105647663No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA179860.1150744820320723RNA PCR Primer, Index 1 (100% over 28bp)
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTA179830.11505528802305993No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC179670.11495291997499404RNA PCR Primer, Index 1 (100% over 24bp)
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT178820.11440908971964399No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG176900.11318067314285327No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT176840.11314228512482856No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG176630.11300792706174208No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG176620.11300152905873795No Hit
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC175630.11236812676133025No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGC174180.1114404163257331No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA172000.11004565167083528No Hit
GTTCGTTGATGAGGCTCAGAGTGAGCGCTGGGTACAG171900.1099816716407941No Hit
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTG169740.10859970299190454No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGA169360.10835657887774805No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTG168530.10782554462840623No Hit
CCCGCCGGGCATGGAATTCTCGGGTGCCAAGGAACTC165920.10615566584433135RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTG164670.10535591546881654No Hit
CAACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA164580.10529833344177948No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA163120.10436422500317821No Hit
GGTCAATGATGTGTTGGCATGTATTATCTGAATCTAT161300.10319978845642866No Hit
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTG160570.10273273423712803No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT160250.10252799814099625No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC158020.10110124347107786No Hit
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGT157610.100838925347909No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC156560.1001671350324766No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG156340.10002637896638598No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)