FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20421874
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG6315633.092581023661198No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA3274981.6036628176238872No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT2163101.0592073969313494No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG2022000.9901148151242144No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG2017570.9879455724778244No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1866430.9139366935669077No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1629210.7977769327144023No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1595580.7813092961008378No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG1368150.669943414595546No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA1301100.6371109722839343No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGA1244670.6094788362713431No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT1239360.606878683121833No Hit
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCG1165030.5704814357389533No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1096410.536880209916093No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGA1095270.5363219849461416No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1071760.5248098191184609No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT1065210.521602473896372No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA1060260.5191786023163203No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC1006850.4930252728030738No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG966770.47339925807004785No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT960320.47024087995058633No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC959090.4696385845882704No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG935340.4580088977142842No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA900580.4409879328410311No Hit
CCCCGGGGAGCCCGGCGGGCTGGAATTCTCGGGTGCC782120.38298150306871936No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT771360.3777126428260208No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT749540.3670280210327417No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT743720.3641781356598322No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGT658930.32265892934213575No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTG639160.31297813315271655No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG631540.3092468399325155No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA627060.307053113734812No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG617500.30237185872364114No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG616010.3016422488944942No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT596150.2919173823127104No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG589820.28881776471640164No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT582740.2853508938503881No Hit
CCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGC572760.2804639770081825No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA549780.2692113368244266No Hit
GGTCAATGATGTGTTGGCATGTATTATCTGAATCTAT538830.2638494390867361No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA524210.25669044868262336No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC492530.24117767057029146RNA PCR Primer, Index 9 (100% over 37bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG490980.24041868047956816No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT487480.23870483188761227No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT462810.22662464766945484No Hit
GTAAATGATGACTTCACTTTTTTCCCCATCAGATCGA458420.2244749918641159No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG454780.2226925893284818No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA453390.22201194660196216No Hit
AGTGATGATGACCCCAGGTAACTCTTGAGTGTGTCGC450130.22041561905631188No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA445310.21805540470967552No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA444370.2175951139449788No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC438270.21460812068471286RNA PCR Primer, Index 1 (100% over 26bp)
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCG437920.21443673582551728No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG432320.21169457807838793No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG429410.21026963539193316No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG427220.20919725584439508No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG421020.20616129548150183No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGT414280.20286091276442114No Hit
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCT412600.2020382654402823No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG411980.20173466940399298No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC410260.20089243523880324No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA404530.1980866202582584No Hit
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG402730.1972052124109668No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC397560.19467361320513485No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG395740.1937824119373178No Hit
GTTCGTTGATGAGGCTCAGAGTGAGCGCTGGGTACAG389830.19088845617204375No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG387900.18994339109133668No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTA386380.18919909113140157No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT370380.1813643547110319No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT360150.17635502011225807No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA359780.176173841832537RNA PCR Primer, Index 1 (100% over 22bp)
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCT355950.17429840180191103No Hit
GGTCAATGATGAGTTGGCATGTATTCTGAATCTAAAG353190.17294690976939728No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT346070.1694604520623328No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG337630.16532762860058778No Hit
TGTAAATGATGACTTCACTTTTTTCCCCATCAGATCG336660.16485264770510286No Hit
TGCCTCTGATGAAGCCTGTGTTGGTAGGGACATCTGA333600.16335425436470719No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGA333010.16306534845920603No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC327880.16055333609442504No Hit
GCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGAT327340.16028891374023754No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT326040.15965234140608253No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTA317770.15560276201880396No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATGG313300.15341393253136318No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGC306510.1500890662629688No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT305800.14974139983431492RNA PCR Primer, Index 1 (100% over 25bp)
ATACATGATGATCTCAATCCAACTTGAACTCTCTCAC303920.1488208183049215No Hit
CTGGATGATGATAAGCAAATGCTGACTTGGAATTCTC303160.1484486683249539No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG298710.1462696322580386No Hit
CCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA296380.14512869876682227No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA288870.14145126935951127No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG287580.14081959373561898No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTC282280.1382243372963715No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC279450.13683856829201865No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG275120.1347182927482561No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG270620.13251477313002716No Hit
ACGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAA268090.1312759054335562No Hit
GGTCAATGATGTAATGGCATGTATTAGCTGAATCTAA262170.12837705295801943No Hit
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGT257440.12606090900374764No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC255680.125199087997507No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG255670.12519419128724424No Hit
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTC250550.12268707563272596RNA PCR Primer, Index 1 (100% over 26bp)
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC243450.11921041134618693RNA PCR Primer, Index 1 (100% over 27bp)
AGAAATGAAGAAACTAAAATTGGTCTTAGTATTGAAG238860.11696282133559338No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA238290.11668370885061773No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC238280.116678812140355No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA234490.11482295895077993RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG234030.11459771027869431No Hit
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC232450.1138240300571828No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGT231280.11325111495644327No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACTGGAA226860.11108676902031615No Hit
CCCTGGCGGAGCTGGAATTCTCGGGTGCCAAGGAACT223340.10936312700783485RNA PCR Primer, Index 1 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG217830.10666503965307003No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTC216300.10591584298287218No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGAT213790.10468676870692671No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG213380.10448600358615473No Hit
ATTCGTGATGACTGATCATTTCTTCACTTTGACCAGA211120.10337934706677752No Hit
GTTCTTGTAGTTGAAATACAACGATGGTTTTTCATAT208720.10220413660372207No Hit
AATACATGATGATCTCAATCCAACTTGAACTCTCTCA208010.10185647017506817No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)