FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19503632
Sequences flagged as poor quality0
Sequence length37
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG3584701.8379653594776602No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC3086491.5825206300036834No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG2835281.4537189791111726No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA2656571.3620898917699023No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG2440751.2514335791405418No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT2381061.2208290230250447No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA1607550.8242310970592553No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT1590990.8157403708191378No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1329790.6818165970317733No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT1242710.6371685027691252No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG1190470.6103837480116524No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1183620.6068715816623284No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT1166860.5982783104193107No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA1157790.5936278945377969No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT1154020.5916949212331324No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1105450.566791867278874No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTC1079340.5534046171502826No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG1061600.5443088753930551No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG987600.5063672243200651No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC966350.495471817761943No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA940470.4822024943866865No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGT926560.4750704894349934No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG914900.469092115765925No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG905340.46419046462730634No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG894330.45854536221766284No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA876050.44917274895260534No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA869450.4457887638569062No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT844160.43282194824020465No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT824190.42258282970064237No Hit
TTCCCTTTGTCATCCTTCGCCTTGGAATTCTCGGGTG793480.40683704450535163No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG764750.3921064548387705No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA748110.3835747105974928No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGA710780.3644346858062129No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG681290.34931442512861194No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC669860.3434539782128785No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC652830.33472227121594583No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA648040.3322663183964915No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT643730.3300564735839971No Hit
GTTCGTTGATGAGGCTCAGAGTGAGCGCTGGGTACAG639510.32789277402280764No Hit
GGTCAATGATGTGTTGGCATGTATTATCTGAATCTAT636080.3261341272230731No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG629540.322780905628244No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATG552830.2834497697659595No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT544140.2789941893899557No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTG539530.2766305270731113No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTG526260.2698266661306981No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA508730.2608385966265155No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG507760.2603412533624506No Hit
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCG498130.25540371147281693No Hit
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC496970.25480895045599716No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCT495680.2541475351872923No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGA493170.25286059540089767No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG476710.24442114166222986No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG473440.24274453086481534No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGA471110.24154988158103063No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGT453390.23246439432409305No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT453220.23237723107162808No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT453210.2323721038214831No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG446860.22911629997940897No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG445060.22819339495330923No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC435760.22342505231846047No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG434960.22301487230686057No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG434090.22256880154424571No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT432790.22190225902539587No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC428960.2199385222198614No Hit
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCT406210.20827402813998952No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGT390810.2003780629166916No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG389740.1998294471511768No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCT387200.19852712561434713No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGC383840.19680436956562758No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC372080.19077472339510917No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGAT368110.18873920508754471No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTC361260.18522703873822066No Hit
GGTCAATGATGAGTTGGCATGTATTCTGAATCTAAAG354850.18194047139527653No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCAC353280.18113549312251176No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT352290.18062789535815688No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT349520.17920764706799228No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG344930.17685423925143787No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA334600.1715577898516543RNA PCR Primer, Index 1 (100% over 22bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTC331760.17010165081047468No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT330650.16953252604437985No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG324530.16639464895564068No Hit
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTC311900.1599189320225074No Hit
TGCCTCTGATGAAGCCTGTGTTGGTAGGGACATCTGA311570.15974973276772245No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC303210.15546335164650357No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGT302460.1550788078856287No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGC300150.153894413102134No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTA293960.15072064526237985No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT290630.1490132709640953No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTA289960.1486697452043804No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGA288200.1477673491788606No Hit
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTC287250.14728026041508577RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGA285820.14654706364435097No Hit
AATACATGATGATCTCAATCCAACTTGAACTCTCTCA282900.14504990660201134No Hit
AGTCTGTGATGAATTGCTTTGACTTCTGACACCTCGT278900.1429990065440119No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG276850.14194792026428718No Hit
TGGAGGTGATGAACTGTCTGAGCCTGACCTTGTAGAA274330.14065585322774754No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC273460.14020978246513266RNA PCR Primer, Index 10 (100% over 37bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA271550.1392304776874379No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG271530.1392202231871479No Hit
ATTCGTGATGACTGATCATTTCTTCACTTTGACCAGA265190.1359695465952188No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC258980.13278552425517462No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGC257410.13198054598240982No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG256030.13127298546240004No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG254490.13048338894007025No Hit
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGT247030.12665846033190126No Hit
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC246090.1261764988182714No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA239060.12257204196633735No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGG237180.1216081189390776No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGAT235050.1205160146581929No Hit
GCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGAT229050.11743966457119372No Hit
GGTCAGTGATGAGCAACATTCACCATCTTTCGTTTGA226710.11623988803726402No Hit
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGT222190.11392237097172465No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG220280.11294306619402991No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCG217460.1114971816531403No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTG217360.11144590915169031No Hit
CCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTG216000.1107486031319705No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG213180.10930271859108087No Hit
GTTCTTGTAGTTGAAATACAACGATGGTTTTTCATAT212910.10916428283716591No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT211940.10866693957310104No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA209200.10726207303337143No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC206000.10562135298697187No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTA203740.10446259445420217No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG202720.10393961493941231No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG202520.10383706993651234No Hit
CTCCATGATGAACACAAAATGACAAGCATATGGCTGA201510.10331921767186747No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA201380.10325256341998248RNA PCR Primer, Index 1 (100% over 28bp)
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCA198220.10163235237416292No Hit
GTACATGATGACAACTGGCTCCCTCTACTGAACTGCC197940.10148878937010296No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTC196970.10099144610603811No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)