FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12788563
Sequences flagged as poor quality0
Sequence length37
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG3269342.5564561084775512No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG2931222.2920636196576583No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG2710982.1198472416330123No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT2676052.092533774123019No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG2104131.6453216831320296No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG2065371.6150133521647427No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1455091.137805709679813No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT986170.771134333075577No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT957770.7489269904679674No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT940030.7350552208250449No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG913350.7141928299528258No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT896340.700891882848761No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG799090.6248473733913654No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT766290.599199456576943No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG748740.5854762571838603No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG697300.5452528169114856No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG683820.5347121486597048No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG680460.5320848010835932No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC556330.4350215110173051No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG514370.40221094426324516No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA494000.3862826495830689No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG493180.3856414516627083No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG488960.38234162821890155No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG446570.34919482353099407No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT435670.3406715828822988No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG417310.3263150050556892No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG401720.31412442508200494No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG370930.2900482251211493No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG355890.27828771692331655No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG347170.27146912440436033No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC341370.2669338220408345RNA PCR Primer, Index 1 (100% over 26bp)
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGG304670.23823630536128257No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA302470.2365160182578762No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT291930.22827427913519294No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG271610.2123850818891849No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG251590.19673046924818682No Hit
CCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA251550.19669919130085217No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGA250160.19561228263097272No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG247810.19377470322506132No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGT247380.19343846529121372No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG242860.18990405724239698No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG239910.18759730862646568No Hit
AGCTACATCTGGCTACTGGGTCTTGGAATTCTCGGGT237970.1860803281807346No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG237370.1856111589707147No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTT233840.18285088011843084RNA PCR Primer, Index 8 (97% over 37bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA232380.18170923504071568RNA PCR Primer, Index 1 (100% over 22bp)
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG230000.17984819717430334No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT227910.17821392442606726RNA PCR Primer, Index 1 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG225880.1766265685988332No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAT221810.1734440374575314No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG214820.1679782161607993No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATGGAAT209750.16401373633613098No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT208700.16319269021859611No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA205780.16090940006316581No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT205500.1606904544318232No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG202260.1581569406977156No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTG195680.15301171836116378No Hit
AACCCGTAGATCCGAACTTGTGGTGGAATTCTCGGGT194310.15194044866495166No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA188710.14756153603809904No Hit
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC188310.14724875656475242No Hit
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGT188110.14709236682807914No Hit
CCCGCCGGGCATGGAATTCTCGGGTGCCAAGGAACTC186860.14611493097387093RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG184180.14401930850244862No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA183920.14381600184477333RNA PCR Primer, Index 1 (100% over 23bp)
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC183340.14336247160842075RNA PCR Primer, Index 1 (100% over 24bp)
CCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTGG180590.14121211272916276No Hit
CCCGGTCAGCCCCTCTCCGGCCCCGTGGAATTCTCGG179930.14069602659814087No Hit
GCCCCGCCGGGGTCGGCCTGGAATTCTCGGGTGCCAA177880.13909303179723945No Hit
CCGCCGGGCATGGAATTCTCGGGTGCCAAGGAACTCC174270.13627019705028626RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG172780.13510509351207012No Hit
ACGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAA171900.13441697867070757No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT169970.13290781771181015No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT167850.1312500865030731No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC167820.1312266280425721No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGT166800.13042904038553824No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTG166440.1301475388595263No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG162470.12704320258656113No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG160770.12571388982483803No Hit
CCCCGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC155140.12131151873748443RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA154980.12118640694814577No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA152200.11901258960838682RNA PCR Primer, Index 1 (100% over 28bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTC151590.11853560091153323No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG151460.11843394758269557No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG149930.11723756609714478No Hit
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTG148810.11636178357177425No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTG148280.11594735076959No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG148240.11591607282225533RNA PCR Primer, Index 2 (97% over 35bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC147740.11552509848057205No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG146760.11475878877087284No Hit
CCCCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGT145160.1135076708774864No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGT144930.11332782268031209No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG144460.11296030679912983No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTC142410.11135731199822842No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC141600.11072393356470153No Hit
CCCCCCTCGCGGCCTGGAATTCTCGGGTGCCAAGGAA138160.10803403009392064RNA PCR Primer, Index 1 (100% over 23bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCT137370.10741629063406107No Hit
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGT136580.1067985511742015No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG134290.10500788868929214No Hit
GCCGGCGGGAGCCCCGGGGATGGAATTCTCGGGTGCC133040.10403045283508398No Hit
CCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGC131110.10252129187618655No Hit
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCC128740.10066807349660786No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAAC128310.10033183556276026RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)