FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20714738
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT5689492.7465903744474103No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG4031171.9460395781978994No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG3812591.8405205028419862No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGG3203001.5462421006724778No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG2903511.4016638781528397No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG2451431.1834231260853987No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA2209341.066554643365511No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT2162241.0438172087911515No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG1989380.9603693756590115No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC1981690.9566570429227731No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1841300.8888840399526173No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG1712630.8267688444816439No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA1681260.8116250372078082No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA1512400.7301081963962084No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC1453950.7018915711123163RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG1386680.6694171077616333No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA1371230.6619586499235471No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC1160190.5600794950918521No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG1159170.5595870920501143No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT1115930.5387130650650759RNA PCR Primer, Index 1 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1099520.5307911690700602No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTC1086660.5245830287595238No Hit
TCACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA975700.47101730178774165No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT851410.4110165429077597No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT777600.37538490711299366No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG771110.3722518720729174No Hit
CCCTGGCGGATGGAATTCTCGGGTGCCAAGGAACTCC742530.3584549319426584RNA PCR Primer, Index 1 (100% over 27bp)
TAGTTCACTGATGAGAGCATTGTTCTGAGCCAGTGGA742120.3582570052298031No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGA663770.320433693151224No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG660530.31886958937158655No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA636390.3072160507171271No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG615580.2971700631695173No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG603950.29155570299754696No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA600470.2898757396786771No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG597300.28834542826464904No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGG596100.28776613056848704No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATTT594860.2871675229491196No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC593920.28671373975379266No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGTGG524530.2532158504732235No Hit
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTC511740.2470415025282965RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG499800.24127749045148436No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG498730.24076095000573988No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG491580.23730930123277444No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT475410.22950326477699112No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC470200.22698814727948766RNA PCR Primer, Index 1 (100% over 24bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTC432720.20889474923602705No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC426370.20582929892716964No Hit
TGGTCATGATGTCAAAACTAAGTTCTGACTTGGAATT410460.1981487769722214No Hit
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC409650.19775775102731205No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA405720.1958605510723814No Hit
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTC405460.1957350365715463No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG398700.19247165954983356No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT393140.18978758022428283No Hit
CCCTGGCGGAGCGTGGAATTCTCGGGTGCCAAGGAAC391200.18885104894882088RNA PCR Primer, Index 1 (100% over 24bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA388840.18771176347970223No Hit
TGTCGCTGATGCCATCACCGCAGCGCTCTGACCTGGA378560.1827491132159142No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT377580.1822760200973819No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTC368710.17799404462658422No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT368400.17784439272174238No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATG367090.1772119927367655No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG366460.17690786144628043No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG364740.17607753474844817No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC361750.17463411798884446No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT356820.17225416995377882No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG356780.1722348600305734No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT355620.17167487225761677No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG350870.1693818188769754No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA349980.16895217308565524No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT345260.16667360214741794No Hit
CACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG326480.15760759320248222No Hit
CTGGACGCGAGCCGGGCCCTTCCCGTGGATTGGAATT319820.15439249098878297No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC307710.14854641173834782No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATTG307180.14829055525587628No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAT305510.1474843659620508No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG286870.13848594174833395No Hit
TTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCAC278970.13467223191526728No Hit
GAATTCACCAAGCGTTGGATTGTTCACCCTGGAATTC274660.13259158768988535No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA272950.13176608847285445No Hit
AAACCTGATGTTACATTGTAGTGTGCTGATGTGGAAT271440.13103713887185056No Hit
AACGAGAACTTTGAAGGCCGAAGTTGGAATTCTCGGG270060.13037094652126424No Hit
AATTCACCAAGCGTTGGATTGTTCACCCTGGAATTCT269650.13017301980840887No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG265230.12803927329421208No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT260870.12593449166482337No Hit
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGGA253160.12221250396698236No Hit
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC252380.12183596046447702No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA251610.12146424444277307No Hit
CGGCCGGGCGCGTGGAATTCTCGGGTGCCAAGGAACT246180.1188429223676399RNA PCR Primer, Index 1 (100% over 25bp)
TTTTATGAGTGAAACATAAGAGTCTGACATGGAATTC240610.11615401556128782No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG234550.11322856219566958No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG229300.1106941347749607No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCA228250.11018724929081894No Hit
CCCCCTCGCGGCCTGGAATTCTCGGGTGCCAAGGAAC226220.10920727068814483RNA PCR Primer, Index 1 (100% over 24bp)
TTCCCTTTGTCATCCTTCGCCTTGGAATTCTCGGGTG223140.10772040660132896No Hit
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC216100.10432186011717841RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)