FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15790475
Sequences flagged as poor quality0
Sequence length37
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA5806143.6769888176258156No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT5534563.5049990579764065No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG3719052.3552489712944036No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG3310472.096498047082181No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG2219771.4057651843912233No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG1482310.9387368017744875No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA1427740.9041779933789198No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG1297950.8219828725861635No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA1248270.7905208678016337No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC1247060.789754583063524No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1239220.784789564595112No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG1192760.7553667638243942No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG1024330.6487011948658922No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG965740.6115965479189195No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG949610.6013815290546991No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG928270.5878670527644039No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGA912790.5780636744619778No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG912610.5779496816910195No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT814980.5161212693095046No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA771590.48864267857680027No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG746330.47264569305229887No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT730710.46275365370579413No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG672460.4258643264372985No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA642920.4071568461366742No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGA621110.39334472205554294No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG618770.39186281603308326No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT601640.38101450399687153No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACT595520.3771387497842845No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG563550.35689236707572125No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGA541370.3428459245209533No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG514610.32589899923846494No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA509840.3228781908080663No Hit
AGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCT496230.31425907073726406No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT473440.29982631934758136No Hit
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC457840.2899469458645164No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA452930.2868374763900389No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG447850.2836203470763229No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA445970.2824297559129792No Hit
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGG442200.28004224065457184No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT422390.26749670291742333No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG410790.26015050212232377No Hit
ATTGGTCGTGGTTGTAGCCCGTGCGAGAATACCATGG405540.2568257129693692No Hit
TTGGTCGTGGTTGTAGCCCGTGCGAGAATACCATGGA397150.2515123832563618No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT378190.23950514471540596No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT373920.23680098287100293No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG372090.23564205636625876No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT367600.23279857002401766No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT353980.22417311702149556No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT351570.22264688047699643No Hit
GAGAAAGCTCACAAGAACTGCTAACTTGGAATTCTCG342510.21690924433875486No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC333610.2112729351080319No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA326400.2067068913379743No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG326120.20652956924981672No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC322630.20431937607956696No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCA311960.19756213793441932No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC311020.19696684235274747No Hit
AGGCAAGATGCTGGCATAGCTGTGGAATTCTCGGGTG310850.19685918251350892No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG310710.19677052146943017No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG308470.19535194476416953No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGG305650.19356605801915394No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA292800.1854282407590652RNA PCR Primer, Index 1 (100% over 22bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC287610.18214144919643013No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA285520.18081786646696824No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG279790.17718909659145782No Hit
CCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCT274870.1740732941852604No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG265310.16801901146102316No Hit
CCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGG258010.1633959713054864No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT251580.15932389620958204No Hit
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTC249380.1579306512312011RNA PCR Primer, Index 1 (100% over 26bp)
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA247220.1565627379796998No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG247070.1564677440039011No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA246040.15581545203674999No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT244450.15480851589328376No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG239220.15149639260376904No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG237780.1505844504361015No Hit
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAA237650.15050212232374263No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGC236300.14964717654155432No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT230050.14568909421660842No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG219240.13884319502738202No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTTGGAATTC218590.13843155446558764No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT217250.13758294161511925RNA PCR Primer, Index 1 (100% over 25bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT216350.13701297776032703No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT216170.1368989849893686No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG216030.1368103239452898No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAA211880.13418215728152572No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGG210090.13304856250366123No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTT209290.13254192796606815No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG208040.13175031150107896No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAA205760.1303064030689387No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGA203610.12894482274915733No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC203200.12868517254864087No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG201730.12775423158581362No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA200820.12717793479930148No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA199820.12654464162731013No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTC198870.12594301311391837No Hit
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTG196440.1244041107059794No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAA195690.12392914082698589No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG192670.12201659544757203No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCT188250.11921743962737029No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCG187890.1189894540854534No Hit
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTG185450.11744421874579454No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACT184480.11682992436896293No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGG184180.11663993641736553No Hit
CTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTTG181470.11492371192126899No Hit
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCT180740.11446140790571531No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGG179950.11396110629984216No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC178820.11324548501549193RNA PCR Primer, Index 1 (100% over 27bp)
TGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACC173360.10978770429641921No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAA172900.10949638943730318No Hit
TAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCC165630.10489234807692611No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG160280.10150422960677244No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT158190.10018064687731053No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)