FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17982121
Sequences flagged as poor quality0
Sequence length37
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG12747417.08893572676994No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT4350312.4192418680755177No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG3641192.024894616157905No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG2812141.5638533407710913No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG2358931.3118196679913343No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT2127161.1829305341677991No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA1620750.9013119197674179No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG1541910.8574683709446733No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATGG1443220.8025860798067146No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT1365860.7595655707132657No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT1354930.753487311090833No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1337150.7435997121807822No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1309730.7283512328718064No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATG1291660.7183023626634478No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT1195570.6648659521310083No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA1074220.5973822554080245No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG975520.5424944031908138No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT954160.5306159379085481No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG802710.44639339263705324No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG743220.41331053216692293No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA742350.4128267182719992No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG727660.40465749285081554No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC722410.4017379262435171No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG689300.3833251928401549No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG655190.3643563515115931No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG634710.3529672612035032No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG613600.34122782290253745No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT593010.3297775607226756No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA568920.3163809208046148No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT540120.30036501255886333No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG527890.293563812633671No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG523570.29116142639680825No Hit
ATTGGTCGTGGTTGTAGCCCGTGCGAGAATACCATGG508300.2826696583790088No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGG503240.2798557522774983No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT501050.27863787592131095No Hit
TTGGTCGTGGTTGTAGCCCGTGCGAGAATACCATGGA412140.22919432029180542No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATTG411040.22858260157408572No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG394670.21947911483856658No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG387430.21545289346012073No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTC379120.21083163660171123No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC374490.2082568569080366No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT371550.20662189960794947No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA355570.1977352949632582No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG354290.1970234768190026No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG353620.1966508845091188No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG333450.1854341876578408No Hit
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC327840.18231442219747046No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG305680.16999107057504506No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC305360.16981311603898117No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG300550.16713823691877058No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG296820.1650639543577757No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT288110.1602202543292863No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA281720.1566667246872602No Hit
TGTCGCTGATGCCATCACCGCAGCGCTCTGACCTGGA280310.1558826125127286No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG277160.15413087254834953No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA276980.1540307731218136No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGA274930.15289075187515422No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG268230.1491648287763162No Hit
GAGAAAGCTCACAAGAACTGCTAACTTGGAATTCTCG262070.14573920395708603No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC256490.1426361217344717No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG255590.14213562460179197No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC251330.13976660484044123No Hit
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGG249720.13887127108086972No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT248150.13799818163830618No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATGGAA247360.13755885637739843No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCATGGAAT246510.13708616464097867No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG239450.13316004268906878No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG236870.13172528424205354No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG233730.12997910535692647No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGGAAT219540.12208793389834269No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTG215890.12005813997136379No Hit
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGT212010.11790044122158892No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT210180.11688276371847348No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA209680.11660470975587361No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG205240.11413559056798696No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG205150.11408554085471896No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC204140.11352387185026727No Hit
TAAAGATTAAGAGAACCAACACCTCTTTACAGTGACC197740.10996478112898919No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG195360.10864124426701388No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT195320.10861899995000589No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG188170.10464282828482803No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG184830.10278542781466103No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACC181240.10078900036319409No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)