FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15148017
Sequences flagged as poor quality0
Sequence length37
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG6356314.196133394885945No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT3412142.252532460189344No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG3233972.134913104467733No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG2292061.5131089435666727No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT2062961.3618680253659605No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG1794081.1843662441097076No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA1768091.1672088828524552No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG1609301.0623832809271339No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG1161060.7664765625758143No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA1153100.7612217493550476No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT1131650.7470614800603934No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCC1047890.6917671138077017RNA PCR Primer, Index 18 (100% over 37bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG999590.6598817521791797No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA823460.5436091073834944No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG738650.48762158109540016No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG732410.48350223002786435No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA707350.4669588105162543No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT655940.4330203748781111No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG642460.42412152032837036No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATGG638690.42163274572506754No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT534980.3531683387997254No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA514920.3399256813614614No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG511280.3375227265720655No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT497670.32853805220841775No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG497280.3282805927666968No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA485650.32060302018409403No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG474520.31325552380882593No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC453690.2995045490112666No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT440890.2910545981035009No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG436210.2879650848028491No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCTG423120.27932368969482935No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAT422090.2786437327077201No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG419830.27715178825056774No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG417510.2756202346485352No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT411580.27170553082954685No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG411430.2716065079673465No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG389770.25730760666561175No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCTGG348910.23033377900222848No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG347480.22938976104925152No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT346670.22885503759336948No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT342190.2258975547756515No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATTT340620.22486111548462084No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG335680.22159996255615502No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA324460.21419305246356668No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT321840.21246345313713338No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG319750.2110837345904748No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC317000.2092683154501345No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATG316800.20913628496720066No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG312930.2065814951224309No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG304500.20101641026676956No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC299890.19797310763514459No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA295960.19537870864549467RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG293380.19367551541564812No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG285400.18840749914658797No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTC282390.1864204403784337No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC277060.1829018280082469No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCCCTG262580.17334282104383697No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTG253280.16720340358741345No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATGGAAT245240.16189577817347311No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT244790.161598709586872No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT243620.16082633126170903No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCT243260.16058867639242813No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG242340.15998133617093246No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG242240.15991532092946556No Hit
ATATGTGATGAATACGTGTTTGGAACTCTGAGGTGGA241110.15916934870088936No Hit
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGG238480.1574331478503094No Hit
AGGCAAGATGCTGGCATAGCTGTGGAATTCTCGGGTG226380.14944530363281214No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG223280.14739883114733762No Hit
AATGGTGGAGTTAAAGACTTTTTCTCTGACCTGGAAT222570.1469301229329225No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG220010.14524013275136938No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG215720.14240807889243853No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT210650.13906110615006573No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC204250.1348361306961829No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT201190.13281606430729515No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC201090.1327500490658282No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA199080.13142314271234315RNA PCR Primer, Index 1 (100% over 28bp)
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG197720.13052533542839304No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACT196100.12945588851662893No Hit
CCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGC196070.12943608394418887No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG190470.12573923042204138No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT190260.12560059841496085No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC189690.1252243115385994RNA PCR Primer, Index 1 (100% over 26bp)
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA189400.12503286733834534No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG188230.1242604890131824No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTG183270.12098613303642318No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG181530.11983746783489878No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT179970.11880763006801484No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTT178010.11751373133526322No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT177770.11735529475574262No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG175440.11581713962956339No Hit
GTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTC175370.11577092896053656No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGG174830.11541444665661518No Hit
TAAAGATTAAGAGAACCAACACCTCTTTACAGTGACC174020.11487972320073313No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG168910.11150634436177355No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGATGGAATT168810.11144032912030663No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC163220.10775007712230585No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA162580.10732757957691756RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAA162090.10700410489372966No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCG161540.10664102106566159No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG160730.10610629760977955No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCCCAT160160.10573001073341812No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCT159880.10554516805731075No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG157580.1040268175035716No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACTGGAA154510.10200014959053716No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG153560.10137300479660143No Hit
TGGTGGAGTTAAAGACTTTTTCTCTGACCTGGAATTC152700.10080527371998593No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA152690.10079867219583924No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)