FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17125271
Sequences flagged as poor quality0
Sequence length37
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG12163697.102772271457777No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT2252321.3152025448239621No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT2220371.2965459057553017No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG1931451.1278361667970103No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG1598070.9331647948812022No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA1463590.854637570406915No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT1419100.8286584194784421No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1350680.7887057670503433No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT1303480.7611441594121342No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATGG1284800.750236302829894No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT1214780.7093493586174491No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1170320.6833877256599327No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG1139940.6656478604046616No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA1000510.5842301707225538No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG980720.5726741492149233No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA927830.5417899664186336No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT916140.5349637970692551No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATG796130.46488607391964776No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG791730.462316771512696No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG773100.45143811154871655No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT738040.43096544282423327No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG710550.4149131421044373No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC700930.4092957127510566No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG696040.40644028348514893No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG685950.4005484059201165No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA664450.3879938600679662No Hit
CCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGC652340.3809224391251969No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA630990.36845548312782905No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG625210.3650803540568789No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG623710.36420445550905445No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG616260.35985415938819304No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG600740.35079152908003614No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTC538990.31473370552793No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG538570.31448845393453917No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT526780.3076038913486391No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT480940.2808364317271242No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGA439530.2566557924835175No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA413660.24154946219537196No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG410520.23971591456859281No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC401090.23420943236460318RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC396910.23176859507799907No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG394820.23054817643469702No Hit
TGTCGCTGATGCCATCACCGCAGCGCTCTGACCTGGA392450.2291642567291344No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT381970.2230446455416676No Hit
CCCTGGCGGAGCTGGAATTCTCGGGTGCCAAGGAACT374800.21885785048306683RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG352620.2059062306225694No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGG352360.20575440820761315No Hit
CCCTGGCGGATGGAATTCTCGGGTGCCAAGGAACTCC350210.20449895362239814RNA PCR Primer, Index 1 (100% over 27bp)
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT338490.19765526630206318RNA PCR Primer, Index 1 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG326710.19077654303981526No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG311800.1820701114744403No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG310770.18146866113826754No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT308540.1801664919638352No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG305400.17833294433705604No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA304460.17778404791375274RNA PCR Primer, Index 1 (100% over 28bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC299710.17501036917897533No Hit
CCTCCCCGGAGGGGGCGGGCTGGAATTCTCGGGTGCC297310.17360893150245624No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT294680.17207318938193736No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC294160.1717695445520249No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC293470.17136663122002566No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTC283740.1656849693064711No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG283720.1656732906591668No Hit
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG278260.16248501994508582No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA276660.16155072816073976RNA PCR Primer, Index 1 (100% over 23bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG264170.15425741291918826No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT258530.15096403437936837No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGGAAT250490.14626921816302935No Hit
CCCCGGGGAGCCCGGCGGGCTGGAATTCTCGGGTGCC248450.1450779961379881No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC247920.14476851198442348No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT243900.142421103876254No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG240400.14037734059799695No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC238830.1394605667846074RNA PCR Primer, Index 1 (100% over 24bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC236860.1383102200251313No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGGAATT236310.13798905722426233No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC233860.1365584229294824RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGATGG231020.13490005501226812No Hit
CCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA229470.13399495984618287No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTG226280.1321322156011429No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT216840.12661989407350108No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG213820.12485641833054788No Hit
CGCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCC213630.12474547118115678RNA PCR Primer, Index 1 (100% over 27bp)
CCGCCGGGCATGGAATTCTCGGGTGCCAAGGAACTCC212000.12379366142585423RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG211950.1237644648075934No Hit
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCA210070.12266667196098677No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA207050.12090319621803358No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG206310.12047108626777352No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATTG204060.11915723844603686No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATGGAAT200140.11686822357438897No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT198110.1156828408729999No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG196270.11460840532100192No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA188880.11029314514205352RNA PCR Primer, Index 1 (100% over 22bp)
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT185970.1085939019592741No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA183890.10737932263962423No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA180960.10566840080954047No Hit
ACCCGCCGGGCAGCTTCCGGGAAACCAAAGTGGAATT174030.10162174951859156No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA172570.10076920826537576No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)