FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25662091
Sequences flagged as poor quality0
Sequence length37
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT5639102.1974436923320084No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG3227121.2575436662585289No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAT2921561.1384730885725562No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG2763091.0767205213324198No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG1804340.7031149566105116No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG1318460.5137773067674025No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA1297950.5057849728613307No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT1252710.48815585604462236No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT1251360.4876297882351052No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG1240990.48358880809829563No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1165620.45421863713288213No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG1142950.4453845947315829No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA1031580.40198594884571176No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT1023650.3988957875646221No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG987720.3848945902342876No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG972070.3787961004424776No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG950590.37042577707327123No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG908680.35409429418670524No Hit
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC905660.3529174610128224No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA874450.3407555526165035No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG853040.33241250683742024No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG803250.3130103466627096No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG775040.30201747784309546No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC753420.2935925992936429No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC663910.25871235512336077No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG640450.24957046563352925No Hit
CTGGACGCGAGCCGGGCCCTTCCCGTGGATTGGAATT624350.2432966199052135No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTG618070.24084943039131146No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG609210.2373968668414433No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAT607130.23658633273492793No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCT604570.23558875229613988No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG585760.22825887415020077No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCA577500.22504011851567357No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG561610.21884810555772716No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG541890.21116361874018763No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGG510520.19893936156644446No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG490980.1913250171235072No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT488450.19033912708048614No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATTGGAA479650.18690994432215208No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG474810.18502389380506834No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC473860.18465369793911182No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTG459860.17919817991448944No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA453410.1766847448245741No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG446300.17391412102778375No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCCATG434400.16927693070685473No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA432440.1685131581834076No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTC428720.16706354910829363No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG428590.16701289072663641No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC422280.16455401081696733No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT401140.15631617859978753No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT395280.15403265462662416No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG394820.1538534018915294No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATGGAAT393650.15339747645661456No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG392790.15306235177795915No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG390200.15205308094340403No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT387140.15086066057516515No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTG385620.1502683471896347No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT383160.14930973473673675No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA369360.1439321526838947No Hit
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTC357480.13930275596014371No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATG355990.13872213297038033No Hit
CCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGG346030.1348409215757204No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG344730.13433433775914833No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG343290.13377319876233No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT341030.1328925222812124No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACT340470.1326743015602275No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG339170.13216771774365543No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCATG338010.13171568910732956No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCT329220.1282904031475845No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG325200.1267238901148001No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTT315940.12311545462137126No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG315840.12307648663548111No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA311290.12130344327747883RNA PCR Primer, Index 1 (100% over 22bp)
AACACATGATATTTGTGGAAATTCTGACTTGGAATTC307470.11981486621647473No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG304800.11877442099320745No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTA302460.11786257012337772No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCG301270.1173988510912848No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA300040.11691954486483583No Hit
TTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCAC292550.11400084272166287No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG289970.11299546868569674No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGG284310.11078988068431367No Hit
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG281400.10965591229491004No Hit
TCACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA275530.10736849152315764No Hit
CTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTTG275200.10723989716972013No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT274910.10712689001063866No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT274760.10706843803180341No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGA271360.10574352651153797No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTC270820.10553309938773112No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG259580.10115309777367713No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACA258680.10080238590066569No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAC257500.10034256366716181No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)