FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27641374
Sequences flagged as poor quality0
Sequence length37
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG7225722.6140958115902633No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG6150762.2252005272965087No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG4363421.578582888101004No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT4256021.5397280902172228No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG2927081.0589488062351748No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG2435460.8810922351399754No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG2332280.8437641341562833No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT2144140.7756995003215108No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT2105410.7616878958332535No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG2069050.7485337016893588No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT2057170.7442357966720468No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG2020630.7310164827551626No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG1673010.6052557300516248No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG1666730.6029837735273218No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA1342880.4858224486235742No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT1278560.46255298307529863No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT1017560.3681293122404118No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG987110.357113217309675No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATTGGAA957870.34653487196403476No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG949820.3436225710053342No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG942530.34098522019925637No Hit
TTCTCCAAAAGAAAGCACTTTCTGTGGAATTCTCGGG875210.3166304251011545No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG844510.3055238860412655No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG788990.2854380538391471No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG740710.2679714836172761No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG724140.2619768467370689No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG722060.26122435158252266No Hit
ACGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAA713590.2581601044868464No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATGGAAT711480.2573967560368019No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC691630.250215492182118RNA PCR Primer, Index 1 (100% over 24bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG653930.2365765175059677No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT640930.23187342279005377No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTG631600.22849804789009404No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA629800.22784685016019826No Hit
TACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGG623500.22556765810556306No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC607690.21984797137797854RNA PCR Primer, Index 1 (100% over 26bp)
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGT606420.2193885152018854No Hit
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTG595070.21528235173837595No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT592590.21438514597718622RNA PCR Primer, Index 1 (100% over 25bp)
TACCCTGTAGATCCGAATTTGTGTTGGAATTCTCGGG586060.21202274532373103No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT583600.21113277509287345No Hit
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTG564490.20421922586048002No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG559630.20246099198976145No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG555510.20097047274133334No Hit
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC553090.20009497357114012No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG539630.1952254616575862No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC538510.19482027195898438No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT524570.18977710731745825No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA520980.18847832962283279RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGC516850.1869841926092386No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG509660.1843830194548216No Hit
CCCCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGT505480.182870793615397No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA501170.1813115368288132RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG475130.17189087633632105No Hit
CCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA472020.17076575136966782RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT469530.16986492784331197No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA464370.16799816101761075No Hit
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG457260.16542592998452246No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC443970.16061792007879203No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGT437920.15842917215330904RNA PCR Primer, Index 23 (100% over 37bp)
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC430250.1556543462709198RNA PCR Primer, Index 1 (100% over 27bp)
ATCTGGAGGTAAGAAGCACTTTCTTGGAATTCTCGGG426090.15414935596182738No Hit
TCTGGACGCGAGCCGGGCCCTTGGAATTCTCGGGTGC416330.15061841715972585No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA408420.14775676491335055No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA404920.1464905471052199RNA PCR Primer, Index 1 (100% over 23bp)
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGT402090.14546671956321708No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC398370.14412091092143248No Hit
CTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTTG390620.14131714291771458No Hit
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCA387990.14036567067903352No Hit
CCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA384980.13927672336404118No Hit
ACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGT377720.13665022585346154No Hit
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGT376510.13621247626836494No Hit
CGGCTCCGGGACTGGAATTCTCGGGTGCCAAGGAACT375860.13597732153256925RNA PCR Primer, Index 1 (100% over 25bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGT365620.13227273000249554No Hit
CCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT363680.13157088356027452RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGG352410.12749366221809377No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC351460.12714997452731547No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG348850.1262057378189666No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGT344420.12460306785038978No Hit
ACCCTGTAGATCCGAATTTGTGTTGGAATTCTCGGGT330280.11948754790554188No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGT325630.11780528710331115No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG322590.1167054864928205No Hit
GCCCCGCCGGGGTCGGCCTGGAATTCTCGGGTGCCAA309790.11207474708022835No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTT302720.10951698710780441No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT302330.10937589426632699No Hit
TACCCTGTAGAACCGAATTTGTGATGGAATTCTCGGG299980.1085257194522964No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG296190.10715458645434918No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC296150.10714011539368483No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG293480.10617417209433945No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA292330.10575812910023938No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG292240.10572556921374458No Hit
CCCGCCGGGCATGGAATTCTCGGGTGCCAAGGAACTC290390.10505628265801838RNA PCR Primer, Index 1 (100% over 26bp)
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA289860.10486454110421572No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG287300.10393839322169729No Hit
ATATGTGATGAATACGTGTTTGGAACTCTGAGGTGGA286410.1036164121219155No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA279010.10093926589901066No Hit
AAGTGCTGTCATAGCTGAGGTCTTGGAATTCTCGGGT278170.1006353736250593No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT277760.10048704525324971No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)