FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28738252
Sequences flagged as poor quality0
Sequence length37
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA16368635.69576395947812No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG12202614.246121162832033No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG6022952.095795527160107No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT4090861.4234895010315867No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG3917861.3632909893058214No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCA3885561.3520516139951728No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG2997411.0430035897799212No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG2963091.031061318552012No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG2782010.9680512231572052No Hit
TCACGCGGGAGACCGGGGTTCGTTTCCCCGACGGGGA2233650.7772393394003225No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA2116450.7364574574681857No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG2011600.6999729837430613No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1772830.616888598513229No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG1743090.6065400219888113No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1741790.6060876632301784No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC1739880.6054230438232638No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG1668700.5806546619467322No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGTGG1537380.53495946795929No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTC1370850.477012310978413No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT1332810.4637755977642621RNA PCR Primer, Index 1 (100% over 25bp)
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC1265650.44040604835673375No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG1259740.43834955584633334No Hit
TAGTTCACTGATGAGAGCATTGTTCTGAGCCAGTGGA1255380.4368324141635337No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC1200840.4178542243975034RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1118240.3891120448105195No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG1112410.38708338976218876No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG1097900.38203437007929364No Hit
CACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG1073560.3735648222445819No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGG1049370.3651474696512509No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1014110.35287810824402266No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG973350.33869492131950124No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT966820.3364226884780605No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCAT954350.3320835240779432No Hit
CCCTGGCGGATGGAATTCTCGGGTGCCAAGGAACTCC938540.3265821456364152RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA823930.2867015015387853No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG767980.26723267650377625No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA744490.2590589017035553No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA722220.2513096481998975No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCT701440.2440788674272882No Hit
TCACGCGGGAGACCGGGGTTCAATTCCCCGACGGGGA673730.2344366665028896No Hit
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTC661330.2301218598820833RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG621950.21641886917826456No Hit
CCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGG621200.21615789297136095No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG616290.214449368736832No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG615050.2140178880747514No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAA591680.20588586946763496No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGG582270.2026114879916844No Hit
GCGGGAGACCGGGGTTCGTTTCCCCGACGGGGAGCCA568310.19775385086051858No Hit
CCCCGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC566410.19709271113636279RNA PCR Primer, Index 1 (100% over 27bp)
CCCCCCTCGCGGCCTGGAATTCTCGGGTGCCAAGGAA563230.1959861720190915RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAT557470.19398187475007178No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG557040.19383224839144705No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT510990.17780830928756558No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCG509970.17745338164617666No Hit
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCC495760.172508752446043No Hit
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC488980.1701495275356344RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTGGA486220.16918913509422912No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG481690.16761283880453134No Hit
TCACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA475110.165323207549297No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA467260.16259165658370595No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC461850.16070914821124124No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGA459290.15981834942501028No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG454610.15818985789393175No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTT453260.15772010072150527RNA PCR Primer, Index 8 (97% over 37bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT450900.15689889559044856No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATGG426040.1482484042522837No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG422860.1471418651350124No Hit
GCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC417710.14534982851427428RNA PCR Primer, Index 1 (100% over 27bp)
CCCCGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACT411370.1431437096452491RNA PCR Primer, Index 1 (100% over 25bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA410820.14295232709351982No Hit
TAACACTTTAGCTCTAGAATTACTCTGAGACCTTGGA387070.13468808054157225No Hit
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGG384060.13364069603119913Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC381550.13276729565876172No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC380940.1325550350104801No Hit
TCTTAGCAGGGCTTGGAATTCTCGGGTGCCAAGGAAC370290.12884917287244887RNA PCR Primer, Index 1 (100% over 24bp)
CCCCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGT369960.1287343433414113No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA367390.1278400648724216No Hit
TCGGGACTGACCTGAAATGAAGAGAATACTCATTGCT366400.12749557627930883No Hit
CCCGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCA365600.12721720165861167RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT364190.1267265663896329No Hit
GCCGGGCGCGTGGAATTCTCGGGTGCCAAGGAACTCC361520.12579749109305605RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT354180.12324340394815941No Hit
CCCCCCACTGCTAAATTTGACTGGCTATGGAATTCTC352990.12282932169987236No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT349090.1214722454239736No Hit
GCCCTCGGCCTGGAATTCTCGGGTGCCAAGGAACTCC349010.12144440796190388RNA PCR Primer, Index 1 (100% over 27bp)
TACTAAAATTGGAACGATACAGAGAAGATGGAATTCT347230.12082502443085265No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTG346340.12051533266532705No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAA337440.1174184150100709No Hit
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGGA335640.11679207211350223No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA330610.11504179168586873No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCG326350.11355944683065623No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG326290.11353856873410394No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC319180.11106451429265775RNA PCR Primer, Index 1 (100% over 24bp)
CCCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGTG317610.11051820409953954No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA316180.11002060946504331RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA315170.10966916150641312No Hit
TCTTAGCAGGGCTTTGGAATTCTCGGGTGCCAAGGAA315050.10962740531330856RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)