FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21403242
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG8462513.953844936201721No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT7280773.4017136282437956No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG3565901.6660560115145173No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG3539971.653941024448539No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG3367351.5732896913467596No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG3364501.5719581173730597No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT3066051.4325166252850854No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG2100740.9815055121088665No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1875380.876213052209567No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG1831920.8559077171579894No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT1659510.7753545000332193No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1624740.7591092975540809No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG1323010.6181353273490062No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA1124270.5252802355830019No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG1107920.5176412059444079No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT1063910.497078900476853No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA1015970.4746804245824067No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT956810.44703975220202624No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG943100.44063418056012266No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATGG915630.4277996763294084No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG878510.41045650934564026No Hit
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGG866040.40463028918703065No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT863080.4032473211301353No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG834290.38979608790107595No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT776680.3628796048748129No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG724380.33844405441007486No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT719870.3363368969990621No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA698290.32625431231399427No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG636010.297155916846616No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT584450.2730661083961019No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA571550.26703898409409194No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG556260.25989520652992665No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG547000.2555687591627474No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA506490.2366417199786836No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG501180.23416078741715857No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG490330.22909146193833627No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG488290.22813833530453No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG482520.22544248203146044No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT481040.22475099800301282No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG467850.21858838020894217No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC458350.21414980029660927No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG447620.20913654109036378No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG434940.20321220495474474No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT418210.19539563211965738No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA418150.19536759898336897No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT407780.190522538594854No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG402120.18787807940497986No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT400180.18697167466498768No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG389770.18210792551894708No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG387760.18116881545328506No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG375100.17525382369642878No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG374540.17499218109107023No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG372740.17415118700241766No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC372710.17413717043427346No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA363970.1700536769149272No Hit
AACACATGATATTTGTGGAAATTCTGACTTGGAATTC345200.16128397744603365No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA342460.1600037975555292RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTG342020.15979822122274748No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT341730.15966272773068677No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG339570.15865353482430372No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGG338590.1581956602649262No Hit
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTG337820.15783590168255818No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC327100.1528273146656941RNA PCR Primer, Index 1 (100% over 27bp)
GAGAAAGCTCACAAGAACTGCTAACTTGGAATTCTCG321900.15039777618736452No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT317920.1485382448135661No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAT305120.14255784240537017No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA301650.14093659269002332RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGT287700.13441888850296604No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC282490.13198467783525505No Hit
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTC279340.13051293818011309No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT272200.1271769949617913No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA270280.12627993460056192No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACTGGAA260840.12186938782451744No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG253320.11835590140970233No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG253030.11822040791764163No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG251400.11745884104847294No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT246690.1152582398498321No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG242580.11333797001407543No Hit
TTTGGCAATGGTAGAACTCACACTGTGGAATTCTCGG235900.11021694750729819No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATG235510.11003473212142346No Hit
TTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCAC232450.10860504217071412No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCA232040.10841348240607661No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA231660.1082359392095833RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTC228690.1068482989633066No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG227810.1064371462977431No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCT227710.10639042440392908No Hit
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGG226820.10597459954898422No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG221530.103503011366222No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAA216850.10131642673572537No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG214420.10018108471604441No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)