FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11768019
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG4832114.10613715018645No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1358501.1543999036711277No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT1278201.0861641198913767No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1253101.064835126455863No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1249821.0620479113774375No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1223211.0394357792930142No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGT1089190.9255508509970964No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1081180.9187442678330142No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG1045770.8886542416357418No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG947140.8048423443232033No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT914490.7770976576431429No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG889910.756210539768843No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT881740.749267994893618No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT835860.710280974223444No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG597900.5080719193264389No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG569270.48374327063883904No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG565290.48036122307416396No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG558980.4749992330909731No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT499940.4248293616793106No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG489600.4160428360967126No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG464140.39440792881112785No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT452970.384916101851977No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG432760.3677424382132626No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG429270.3647767733889621No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT426870.3627373477218213No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG417570.3548345732616509No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC410460.34879277472274645No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG384370.32662251819953725No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC375710.3192635905839377RNA PCR Primer, Index 1 (100% over 24bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT360570.30639821366705816No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC345610.2936857936752142RNA PCR Primer, Index 1 (100% over 27bp)
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT333250.2831827514894393RNA PCR Primer, Index 1 (100% over 25bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTC332480.282528435754565No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC331000.28127078992649485No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG323970.27529697224316174No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG321740.2734020058941101No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA315880.26842240822350816RNA PCR Primer, Index 1 (100% over 28bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA286520.24347343422881965No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG280270.23816242988730726No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA280210.23811144424562874No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG277830.23608901379238087No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG275600.23419404744332925No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG264710.2249401534786781No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT250450.21282256597308347No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG234670.19941334221163307No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC225470.1915955438209269No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG215740.18332737226206042No Hit
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCT214030.18187428147422263No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA212380.1804721763280634RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT205600.17471079881839077RNA PCR Primer, Index 16 (100% over 37bp)
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGT204620.17387803333764162No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG204410.1736995835917668No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG204010.17335967931391003No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAA199830.16980767961030654No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG195360.1660092493052569No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTC195090.16577981391770355No Hit
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGT189320.16087669470961935No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGGAATTCT186460.1584463791229433No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG186400.15839539348126477No Hit
GGACAGAAACTGTGTGCTGATTGTCACGTTCTGATTT182540.15511531719994673No Hit
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC173940.14780737522602572RNA PCR Primer, Index 1 (100% over 27bp)
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTC172240.14636278204513437RNA PCR Primer, Index 1 (100% over 26bp)
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA169760.14425537552242226No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT167160.1420459977163531No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG164910.14013403615340866No Hit
CTGGATGATGATAAGCAAATGCTGACTTGGAATTCTC162320.13793315595428596No Hit
CCCTGGCGGATGGAATTCTCGGGTGCCAAGGAACTCC162070.13772071578062545RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA160500.13638659149003754No Hit
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGG160010.13597020874966298No Hit
AGCTACATCTGGCTACTGGGTCTTGGAATTCTCGGGT159780.1357747637898953No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA158200.134432141892361RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTG156870.13330196016848717No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC156260.13278360614475554No Hit
TCGAGGAGCTCACAGTCTAGTATGGAATTCTCGGGTG153060.13006437192190123No Hit
CCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT152460.129554515505116RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT151740.12894268780497378No Hit
GCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG151280.1285517978854385RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTG149620.12714119513233282No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT146210.12424351116360366No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT142890.1214223056573923No Hit
TGAATCTGACAACAGAGGCTTACGACCCCTTATTTAC140690.11955283212917993No Hit
CCCGGTCAGCCCCTCTCCGGCCCCGTGGAATTCTCGG138900.1180317604857708No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT138120.11736894714395006No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGT136140.11568642096855895No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG135660.1152785358351308No Hit
TTCTGTGATGAAAATGTTTCTGAAGTGGAATTCTCGG134690.11445426796132807No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG133510.11345155034165053No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG133310.11328159820272214No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG131980.11215141647884831No Hit
AGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCT130440.11084278500909966No Hit
CGGCTCCGGGACTGGAATTCTCGGGTGCCAAGGAACT130220.11065583765627843RNA PCR Primer, Index 1 (100% over 25bp)
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT129900.11038391423399299No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA129390.10995053627972558No Hit
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC129260.10984006738942213No Hit
CCCTGGCGGAGCGTGGAATTCTCGGGTGCCAAGGAAC129070.10967861285744016RNA PCR Primer, Index 1 (100% over 24bp)
CGGCGCGACCGCTGGAATTCTCGGGTGCCAAGGAACT128370.10908378037119076RNA PCR Primer, Index 1 (100% over 25bp)
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC128030.10879486173501249RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC127520.10836148378074509No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGT127410.10826801010433446No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG123440.10489446014660582No Hit
ATCAATGATGAAACTAGCCAAATCTGAGCTGGAATTC122200.10384075688524975No Hit
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT121520.1032629196128932RNA PCR Primer, Index 1 (100% over 30bp)
CCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA121000.1028210440516794RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCC120550.1024386517390905No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG120540.10243015413214408No Hit
TGTAAACATCCCCGACTGGAAGCTTTGGAATTCTCGG120190.10213273788901937No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)