FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16479992
Sequences flagged as poor quality0
Sequence length37
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT2676301.6239692349365218No Hit
CCCCGGGGAGCCCGGCGGGCTGGAATTCTCGGGTGCC2386351.4480286155478717No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG2361881.4331803073690812No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG2201451.3358319591417276No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG2080641.262524884720818No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1910691.159399834660114No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT1654711.0040720893553832No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1626130.9867298479271107No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG1411300.8563717749377548No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG1401730.8505647332838512No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT1264580.7673426055061191No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG1066150.6469359936582494No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC1044500.6337988513586656RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1032960.6267964207749616No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT822210.4989140771427559No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT810020.49151722889185867No Hit
CCCTGGCGGAGCTGGAATTCTCGGGTGCCAAGGAACT718090.4357344348225412RNA PCR Primer, Index 1 (100% over 25bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT712850.4325548216285542No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG648030.3932222782632419No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG641320.3891506743449875No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG618320.3751943568904645No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC514660.31229384091934026No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG473250.2871664015370881No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA470570.28554018715543067RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG449790.27293095773347464No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT443330.2690110529179869No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG437470.26545522594913884No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG432770.2626032828171276No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA429430.2605765828041664No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC396830.24079501980340767RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG396050.240321718602776No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGT389030.2360620077970912No Hit
CGGCCGGGCGCGACTGGAATTCTCGGGTGCCAAGGAA386770.2346906479080815RNA PCR Primer, Index 1 (100% over 23bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCT356160.21611660976534455No Hit
CCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTTG337870.2050183034069434No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA336800.20436903124710254RNA PCR Primer, Index 1 (100% over 23bp)
ACGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAA332160.2015534958997553No Hit
CCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCC331740.2012986414071075RNA PCR Primer, Index 1 (100% over 27bp)
CCCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA315280.1913107724809575RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG314870.19106198595242035No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG311600.18907776168823384No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA310850.18862266437993416No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATTT306840.18618941077155862No Hit
CCTCCCCGGAGGGGGCGGGCTGGAATTCTCGGGTGCC296410.17986052420413795No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG293010.1777974164065128No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG287720.1745874633919725No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG266780.1618811465442459No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT265300.1609830878558679RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG264730.16063721390156016No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGC255730.15517604620196418No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT254780.1545995896114513No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG252650.15330711325588023No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGA250330.1518993455822066No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC250290.15187507372576395No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA245460.1489442470603141RNA PCR Primer, Index 1 (100% over 22bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT242330.14704497429367686No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGT240970.14621973117462678No Hit
ACCTCACCCGGCCCGGACTGGAATTCTCGGGTGCCAA232320.140970942218904No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT232120.14084958293669075No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG230450.13983623293021016No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTT225930.13709351315219087No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA225480.13682045476721105No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT225410.1367779790184364No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC225150.1366202119515592No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTG224250.1360740951815996No Hit
CCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAAC216940.13163841341670554RNA PCR Primer, Index 1 (100% over 24bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG215910.13101341311330733No Hit
CCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT211270.1281978777659601RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTG208620.1265898672766346No Hit
TCGAGGAGCTCACAGTCTAGTATGGAATTCTCGGGTG205980.12498792475141979No Hit
CCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG202120.12264569060470418RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGG200460.12163840856233424No Hit
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGT199680.1211651073617026No Hit
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC199000.12075248580217758No Hit
CCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG198210.12027311663743526RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAG196730.11937505794905727RNA PCR Primer, Index 3 (97% over 37bp)
CCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA196560.119271902559176No Hit
GCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT193550.11744544536186668RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG186920.11342238515649765No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTC186840.11337384144361234No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATT185360.11247578275523434No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG179940.10918694620725544No Hit
CGGCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCC178050.1080401009903403RNA PCR Primer, Index 1 (100% over 27bp)
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT175080.10623791564947363No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG174290.10575854648473129No Hit
CCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA173010.10498184707856653RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGT172540.10469665276536541No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA167760.10179616592046889No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC167460.10161412699714902No Hit
AACCCGTAGATCCGAACTTGTGGTGGAATTCTCGGGT166380.10095878687319752No Hit
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCA165780.10059470902655779No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG165340.10032771860568863No Hit
TTCTGTGATGAAAATGTTTCTGAAGTGGAATTCTCGG165250.10027310692869269No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)