FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24282106
Sequences flagged as poor quality0
Sequence length37
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT5190752.137685256789506No Hit
CCCCGGGGAGCCCGGCGGGCTGGAATTCTCGGGTGCC3518851.4491535454132356No Hit
GCCCCGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAG2385480.9824024324743497No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG2317580.9544394543043342No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG2099680.8647025921063025No Hit
CCGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAGGAA1989000.8191217022114968RNA PCR Primer, Index 1 (100% over 23bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT1932800.7959770869956667No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT1864310.7677711315484744RNA PCR Primer, Index 1 (100% over 25bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA1828790.7531430758106402No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1820270.7496343191978488No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG1715910.7066561689500902No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG1685210.6940131140190229No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1330280.5478437496319306No Hit
CGGCTCCGGGACTGGAATTCTCGGGTGCCAAGGAACT1222430.5034283270157869RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG1146530.4721707416976106No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1102770.4541492405971706No Hit
ACGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAA982500.4046189403835071No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG855860.35246530922812047No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG816940.33643704545231784No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG770880.3174683447967816No Hit
CCCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA720320.2966464276204049RNA PCR Primer, Index 1 (100% over 28bp)
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCA678370.2793703313872364No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA635950.26190067698411335No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA621480.25594155630487736No Hit
CCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA615190.25335117143463587RNA PCR Primer, Index 1 (100% over 28bp)
GCCCCGCCGGGGTCGGCCTGGAATTCTCGGGTGCCAA586420.2415029404780623No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA519040.2137541117726774No Hit
CCCTGGCGGAGCTGGAATTCTCGGGTGCCAAGGAACT487530.2007774778678587RNA PCR Primer, Index 1 (100% over 25bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG469720.19344285870426559No Hit
CGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAG467170.1923927026757893No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC457110.18824973418697702RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT453940.18694424610451826No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT451040.18574995101330996No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATTGGAA450660.18559345717377232No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG449570.18514456694983542No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG445570.18349726337575498No Hit
TGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACC433530.17853887961777284No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTT428470.1764550405965611No Hit
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTC427140.17590731215817937RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA420060.17299158483205698No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG419560.17278567188529695No Hit
CCCGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAGGA416100.17136075429371736RNA PCR Primer, Index 1 (100% over 22bp)
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG415300.1710312935789013No Hit
CCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA414340.17063594072112195RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA406050.16722190406384024No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT397660.16376668481720655No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG396790.16340839628984405No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA386280.15908010614894771No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC384290.1582605726208427RNA PCR Primer, Index 1 (100% over 26bp)
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAA378200.15575255292930523RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAGGAAC377710.15555075824148037RNA PCR Primer, Index 1 (100% over 24bp)
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG372380.1533557262290182No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCC368770.1518690347534106RNA PCR Primer, Index 18 (100% over 37bp)
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA368130.15160546618155774RNA PCR Primer, Index 1 (100% over 23bp)
CCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAACTC363340.1496328201515964RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT357130.14707538135283654No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG349660.1439990419282413No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA349590.1439702141156949RNA PCR Primer, Index 1 (100% over 22bp)
CCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAAC349570.1439619775978245RNA PCR Primer, Index 1 (100% over 24bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA348280.14343072219518357No Hit
GCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC347400.14306831540888587RNA PCR Primer, Index 1 (100% over 24bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG341090.14046969402077397No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT338670.13947307535845532No Hit
CCCCCGGGGAGCCCGGCGGGCTGGAATTCTCGGGTGC331290.1364338002642769No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC330150.13596431874566398No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG328270.13519008606584618No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG320170.1318542963283333No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGT317560.13077943074624582No Hit
CCCGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCA314520.12952748002994469RNA PCR Primer, Index 1 (100% over 28bp)
CCCCGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAGG314450.12949865221739829Illumina Small RNA Adapter 2 (100% over 21bp)
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG308990.12725008283877848No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT304830.12553688712173483No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG300530.12376603577959835No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG299710.12342833854691188No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT293990.12107269443597685No Hit
CCCCGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACT293020.12067322331926235RNA PCR Primer, Index 1 (100% over 25bp)
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC290350.11957364818356364RNA PCR Primer, Index 1 (100% over 24bp)
CCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC283220.11663732956276526RNA PCR Primer, Index 1 (100% over 27bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAA282750.11644377139281081No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG281820.11606077331183713No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG278540.11470998438109117No Hit
GCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC278310.11461526442558155RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGCCGCCGGTGAAATACCACTACTCTGATGGAAT271770.11192192308196003No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC270830.11153480674205112No Hit
CCCTGGCGGATGGAATTCTCGGGTGCCAAGGAACTCC269130.11083470272306693RNA PCR Primer, Index 1 (100% over 27bp)
CCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGC266830.10988750316797069No Hit
GTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCC261340.10762657901254527No Hit
CCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCC258670.1065270038768466RNA PCR Primer, Index 1 (100% over 27bp)
CCGCCGGGGTCGGCCCTGGAATTCTCGGGTGCCAAGG257660.10611105972439128Illumina Small RNA Adapter 2 (100% over 21bp)
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC256840.1057733624917048No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT255780.10533682704457349No Hit
GCCGGCGGGAGCCCCGGGGATGGAATTCTCGGGTGCC255120.10506502195485022No Hit
CCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG253750.10450082048072766RNA PCR Primer, Index 1 (100% over 29bp)
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC250470.10315003154998172No Hit
CGGCCCTGGCGGAGCTGGAATTCTCGGGTGCCAAGGA249050.10256523878118315RNA PCR Primer, Index 1 (100% over 22bp)
CTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTTG248540.1023552075754879No Hit
TTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCAC246250.10141212627932684No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)