FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18362473
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG7627664.1539393958537065No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG7229943.937345476287426No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA4802592.6154374740265105No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA4295022.3390204576475075No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGA3147621.71415908957361RNA PCR Primer, Index 19 (100% over 37bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG3080101.6773884432668738No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG2345611.2773933010003609No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG2326021.2667248033530123No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT2297701.2513020441200922No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT2092131.1393508924424287No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT2045441.1139240340884367No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG1888351.028374554995942No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG1880941.0243391508322435No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT1837521.0006930983642561No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1756840.9567556613970243No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA1665920.9072416335207136No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT1591480.8667024316387014No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG1304110.7102039033631254No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG1241220.6759547039225055No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA1228790.6691854631998643No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG1161340.6324529381200452No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG1150160.6263644335923598No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG963090.5244881776000433No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA897080.48853986061681337No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG844560.45993804864954735No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA839030.45692647172306255No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATGG822110.44771202658813986No Hit
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGG720020.3921149400737036No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG714140.3889127570126311No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT636140.3464348184473845No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA599060.3262414599602134No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA578700.3151536288167721No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACT574520.3128772469808397No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG564270.3072952101822015No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG550250.2996600730195764No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG542470.295423170942171No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT539530.29382207941163485No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAA520390.28339864679447047No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG517040.2815742737919887No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA488390.26597180020360006No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTG473370.2577920740850102No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA470250.2560929565424004No Hit
ATATGTGATGAATACGTGTTTGGAACTCTGAGGTGGA453930.24720526478105645No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTG452420.24638293545754977No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT437660.23834480246751077No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT431780.23514261940643832No Hit
CCCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA430020.23418414284394046RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCT426510.23227263560850436No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGC419380.22838971635247607No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA416340.22673416592634338No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT411760.22423994850802503No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG409940.2232487966081693No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT392030.2134952084068415No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG387570.21106634166323893No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC383500.20884986461246258No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA381880.2079676305037998RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA367050.19989137628684325No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG364740.1986333757985648No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG358650.1953168290567398No Hit
TACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGG348340.18970211692074374No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG339040.18463743963027202No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTC335080.18248086736465183No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA332210.18091789706103312No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG325550.1772909346143082No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG325460.1772419216082714No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA320080.1743120330251813No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG312760.1703256418675197No Hit
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGT306420.16687294788670348No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG304620.16589268776596705No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC302860.16493421120346916No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC289800.15782187943857018No Hit
TACCCTGTAGATCCGAATTTGTGTTGGAATTCTCGGG279700.1523215309833267No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTG277770.15127047429831483No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATG276690.15068231822587297No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGTGG275310.14993078546664168No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG274220.14933718350464015No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATT272350.14831880215698615No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG261720.1425298215550814No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGA257580.14027522327738753No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATT245510.1337020345788936No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG245130.133495090775627No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGATG241260.1313875315160436No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGGAA240580.13101721102598762No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGA233710.12727588489851013No Hit
CCCCGGGGAGCCCGGCGGGCTGGAATTCTCGGGTGCC231180.12589807483991944No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTG226180.12317513006009594No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA225650.12288649791343464No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGA225530.12282114723871887No Hit
AGCTACATCTGGCTACTGGGTCTTGGAATTCTCGGGT223950.12196069668829465No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG222210.12101311190491609No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAAT220080.11985313742871126No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC217840.11863325816735035RNA PCR Primer, Index 1 (100% over 27bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGA217600.11850255681791881No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA217400.11839363902672587No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC209720.11421119584491697No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG209560.11412406161196262No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT208670.11363937744115404No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG207970.11325816517197874No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC203060.11058423339819205No Hit
TTACATGATGAATACGTGTCTGGAACTCTGAGGTGGA189330.10310702703279673No Hit
AGGCAAGATGCTGGCATAGCTGTGGAATTCTCGGGTG187990.10237727783180403No Hit
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGT187060.10187081010275686No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAAT185590.10107026433748875No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATGGAA184610.10053656716064335No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA183940.10017169256014699No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC183680.10003009943159617RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)