FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9973790
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG3108773.116939498425373No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1460321.4641575569567837No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT1328561.3320513064742692No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1258261.2615665659694058No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT1238261.241514008215533No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1212001.2151849998846977No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG922280.9247036482620951No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGT874770.8770687973177699No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT874040.8763368789597535No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG798000.8000970543795288No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG796270.7983625081338187No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG686240.688043361650887No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG574230.5757390119503218No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT516730.5180879084079372No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG491720.4930121849367191No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG488710.4899942749947613No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG442330.44349239356353004No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC426050.42716961155187744No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT420790.4218957888626089No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG405820.40688644938383506No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG405100.40616455730469564No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG376050.3770382171671952No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC342080.3429789478222421No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC334610.33548931750117056No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG333450.3343262691514459No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG313120.31394284419463414No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT293060.29383012876749964No Hit
CAATCTGAATACGAGGGTCCGATTCCCTTCATCCGCA290570.29133358532714243No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT270230.2709401340914537No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG267450.26815282856366535No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT266060.2667591757997712No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA265730.2664283085968323RNA PCR Primer, Index 1 (100% over 28bp)
GGACAGAAACTGTGTGCTGATTGTCACGTTCTGATTT263860.26455339444684517No Hit
CCCGGTCAGCCCCTCTCCGGCCCCGTGGAATTCTCGG263340.2640320279452445No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC262080.2627687168067505No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG257130.25780570876266695No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTC254990.25566008508300253No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG252310.25297304234398355No Hit
CCCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA243400.2440396278646332RNA PCR Primer, Index 1 (100% over 28bp)
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG237440.23806396565397908No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTC237260.23788349263419423No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT233720.23433418991175872No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG230500.2311057281133852No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGG218860.21943513950063118RNA PCR Primer, Index 17 (97% over 37bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT202670.20320259399887103No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG198080.1986005319943572No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT194120.19463012555909037No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGT190350.19085021842248534No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA190250.19074995563371597No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG186980.18747136244095775No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG181780.1822576974249508No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA180030.18050309862148692No Hit
CCGGTCAGCCCCTCTCCGGCCCCGTGGAATTCTCGGG179580.18005191607202478No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC176090.17655274474397395RNA PCR Primer, Index 1 (100% over 24bp)
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG175510.17597122056911163No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC168580.16902300930739467RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA165680.1661153884330831No Hit
TGTCGCTGATGCCATCACCGCAGCGCTCTGACCTGGA164880.1653132861229282No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA159190.15960833344195136No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG158660.1590769406614737No Hit
CGGCTCCGGGACTGGAATTCTCGGGTGCCAAGGAACT157160.15757299882993325RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG155120.1555276379390382No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT155030.15543740142914578No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG153020.15342211937488157No Hit
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGT149470.14986279037356914No Hit
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGG149320.14971239619041507No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT148650.14904063550566032RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTG146970.147356220654335No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA144080.1444586260589004RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA142440.14281431632308278No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC142420.14279426376532894No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTG139600.13996685312203286No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG139540.1399066954487712No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT134010.1343621632298254No Hit
AGCTACATCTGGCTACTGGGTCTTGGAATTCTCGGGT132660.13300861558143895No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG131820.1321664081557763No Hit
GCGGATGTAGTTCAATGGTAGAACATCAGGTTGCTGG130420.13076272911300518No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG128800.1291384719349415No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT127390.12772476661329343No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG124200.12452638365155072No Hit
CGCAGGTTCGAATCCTGCCACGATCGCCATGGAATTC120110.1204256355908837No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT117970.11828001191121931No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA116880.11718714751363322No Hit
TGAGAACTGAATTCCATGGGTTGTGGAATTCTCGGGT116770.11707685844598693No Hit
TGTTGCTGATGCCATCACCGCAGCGCTCTGACCTGGA116430.11673596496417109No Hit
CCCGGGGCTATGGAATTCTCGGGTGCCAAGGAACTCC116030.11633491380909364RNA PCR Primer, Index 1 (100% over 27bp)
GCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG115960.11626472985695507RNA PCR Primer, Index 1 (100% over 29bp)
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTC114090.11438981570696798RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC112610.11290592643318138No Hit
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCA112180.1124747964414731No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA112000.11229432342168824No Hit
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC108520.10880517837251437RNA PCR Primer, Index 1 (100% over 27bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT108300.10858460023722176No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA105910.10618831958563393No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG105700.10597776772921828No Hit
CTCACTGATTACTTGATGACAATAAAATATCTGATAT104570.10484479821612445No Hit
TCTCACTGATTACTTGATGACAATAAAATATCTGATA103940.10421314264687748No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG103710.10398253823270794No Hit
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGT103500.10377198637629226No Hit
GAGAAAGCTCACAAGAACTGCTAACTTGGAATTCTCG102980.10325061987469157No Hit
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCT102790.10306012057602978No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG101090.10135565316695058No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG100730.10099470712738087No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG100030.10029286760599532No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)