FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21483680
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA15660197.2893424217824885No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG11683885.438490984784729No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA5495872.558160426891482No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA5309852.471573771346436No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT4061791.8906397786598945No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA3169191.4751616110461523No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG2973831.3842274694093377No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA2939331.3681687681067676No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA2375741.1058347545671876No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG2348801.093295003463094No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG1924210.8956612647367677No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG1707230.7946636702836758No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG1560980.7265887408488676No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA1500830.6985907442300388No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT1500780.6985674707498901No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA1454220.676895206035465No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG1286620.5988825005771823No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACT1283680.5975140199444415No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA1271650.5919144206206758No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1208770.5626456919857306No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT1105650.51464646652715No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT1016510.4731545061181325No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG930370.4330589545180341No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG849540.3954350465097227No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT843160.39246535044275466No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC841530.39170663498990865No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG802960.37375347240323814No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGA771470.3590958346056169No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA749210.34873448124343687No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGA742290.3455134315908634No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG739180.3440658211256172No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG715690.3331319401517803No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA691300.32177913653526774No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG678870.3159933493703127No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT645310.30037218949453726No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT623720.29032270076634914No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAA623660.2902947725901708No Hit
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCT609810.28384801858899406No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG602450.28042216231111244No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGA588380.2738730049972817No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG575410.26783586424672123No Hit
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC554880.2582797732976846No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC541170.2518981850409241No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC537150.25002699723697247No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT533120.24815115473699106No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT528870.2461729089243556No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAA527130.2453629918151825No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG523870.2438455609094904No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT508310.23660285388722976No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGA485680.22606927677194968No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGATG484880.22569690108957124No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC475110.22114926306852456No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAAT449610.20927978819271187No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA449160.20907032687137397No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG416620.19392394599063104No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG411470.19152677753531983No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGA400480.18641126659864604No Hit
CCCGGTCAGCCCCTCTCCGGCCCCGTGGAATTCTCGG392600.18274336612721842No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG381710.17767440215084193No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG380100.17692499609005535No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC377290.17561702650570107No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA368190.17138125311864635RNA PCR Primer, Index 22 (100% over 37bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAA367790.17119506527745712No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTC362630.16879324212611618No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAA353980.16476693006039933No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT351110.1634310322998667No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG348890.1623976897812665No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATG342130.15925111526516872No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGG326580.15201306293893782No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG308480.14358806312512568No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG302280.14070215158669278No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGA301430.14030650242416567No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT294040.13686668205819486No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG291420.1356471516984055No Hit
CTGGATGATGATAAGCAAATGCTGACTTGGAATTCTC288990.134516060563181No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCG275750.12835324301981782No Hit
TGAGAACTGAATTCCATGGGTTGTGGAATTCTCGGGT273030.12708716569973114No Hit
GTTGCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA272730.12694752481883922No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCGTGGA271670.1264541270396878No Hit
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGA264480.12310740059431159No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGG254720.11856441726929466No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCTGGA254280.11835961064398651No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGGAATTCTC250130.11642791179164835No Hit
AGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCT248670.11574832617130772No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGA247430.11517114386362112No Hit
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCTTGGA243270.11323479031525324No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAAT236680.11016734563166086No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT227130.10572211092326828No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGT224930.10469807779672756No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTG220370.10257553640717046No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)