FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10756575
Sequences flagged as poor quality0
Sequence length37
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG5401085.021189365574079No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG2220772.0645698096280647No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1878561.746429509393092No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT1801921.6751800642862622No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1443711.3421651408557091No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT1441051.339692234749444No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1434761.3338446485056814No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1211741.1265109944382854No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA932170.8666048440140101No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG915700.8512932787620595No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC911170.8470819010698108No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT795680.7397150115162122No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG792070.7363589246577094No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA733320.6817411676114377No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT678050.6303586411102047No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG610730.5677736640148002No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG607500.5647708494571925No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATGG586030.544810964456623No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC545850.5074570669567218No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG488370.45401998312659936No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG483790.44976212223686446No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG467740.43484101584379786No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG456490.4243822964094054No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG454940.4229413172873336No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG452680.4208402767609578No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG449120.41753067309994124No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG444980.4136818643480848No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG422280.39257849268935513No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG407030.3784011174560676No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA402690.3743663759142664No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG397840.3698575057581061No Hit
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGG366950.3411401863511387No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG350890.3262097833185749No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT348440.32393210664175165No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG342910.3187910649997792No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA333270.309829104524442No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT330160.30693784964080106No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG325710.3028008450645303No Hit
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTG322390.2997143607514474No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG320920.2983477547453534No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC318490.29608867134752465No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGT308200.28652242930486704RNA PCR Primer, Index 23 (100% over 37bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTG295900.27508756272326457No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG284220.2642290877904909No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT280170.26046394879410967No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG278980.25935764869393835No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG265130.2464818029902641No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT262870.24438076246388837No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATGG260920.242567917761927No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC257650.23952791664633025RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA253630.23579066756844072No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG244200.22702393652254552No Hit
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGT239230.22240350669241837No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA236810.22015371993408683No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA236790.22013512665509236No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT236750.22009794009710343No Hit
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCC231420.21514283124507572No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG230660.21443628664328562No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG223890.20814246170365566No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT221890.2062831338042081No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAA215950.2007609299428489No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA211490.19661462872708088RNA PCR Primer, Index 1 (100% over 22bp)
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT210880.1960475337177494No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT207650.19304471916014157No Hit
AGCTACATCTGGCTACTGGGTCTTGGAATTCTCGGGT206290.19178037618851726No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC206030.19153866356158908No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG202690.18843358596951168No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT198530.18456618393866078No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG196880.18303223842161656No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG192750.17919272630925737No Hit
AGGCAAGATGCTGGCATAGCTGTGGAATTCTCGGGTG189900.17654318405254463No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA188820.17553914698684295RNA PCR Primer, Index 1 (100% over 28bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTC188240.17499994189600315No Hit
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGT185750.17268507866119095No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT182220.16940336491866603No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT180800.16808324211005826No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG178310.16576837887524606No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG177920.1654058099348538No Hit
GGACAGAAACTGTGTGCTGATTGTCACGTTCTGATTT170060.15809865129002493No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGT168150.15632299314605252No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA167680.15588605108968234No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA164240.15268800710263256No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC164070.15252996423117954No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC163880.15235332808073201No Hit
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGG159390.14817913694647228No Hit
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC158670.14750977890267117No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC157360.146291919128533No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGGAATTCTC156690.14566904428221808No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT154250.14340066424489206No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACT152040.14134610691600252No Hit
GTGAATGATGAACATGAACTTTCTGACCTGGAATTCT151450.1407976051856655No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA146670.13635381150598586No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT146120.1358424963336378No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATG144620.13444800040905214No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA139910.13006928320585318No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG136920.12728958799617907No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC133440.12405435745114035No Hit
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGT132690.12335710948884752No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG131910.12263197160806297No Hit
TTTGGCAATGGTAGAACTCACACTGTGGAATTCTCGG130130.12097716977755467No Hit
CTGGATGATGATAAGCAAATGCTGACTTGGAATTCTC129560.1204472613262121No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTC129320.1202241419782784No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGC128600.11955478393447729No Hit
TCTCAGTGATGTAATTCCAATAGATCCTTCTGACCTG126240.11736077701312918No Hit
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTG125880.11702609799122864No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG125830.11697961479374243No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG125210.11640322314491369No Hit
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCT124810.11603135756502417No Hit
ATTAATGATGAGATATAACCTTGACTGAAGTGGAATT123700.1149994305808308No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGATGG123040.11438585237401311No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGT122770.11413484310758767No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGT122730.11409765654959875No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG121720.11315869596037773No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG119700.1112807747819357No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG119270.11088101928355448No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC119200.11081594280707383RNA PCR Primer, Index 1 (100% over 31bp)
CCCGGTCAGCCCCTCTCCGGCCCCGTGGAATTCTCGG118740.11038829739020088No Hit
CCCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA116720.10851037621175885RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAAT116200.10802695095790249No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGC114520.10646511552236655No Hit
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTG111900.10402939597409028No Hit
TCGAGGAGCTCACAGTCTAGTATGGAATTCTCGGGTG111900.10402939597409028No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGC111870.10400150605559856No Hit
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGC111550.10370401359168695No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCT111430.10359245391772008No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTG110830.10303465554788584No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGA110250.10249545045704603No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)