FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14346242
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA10073327.021574012204729No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT6675764.653316178550453No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG6620674.614915878318517No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA6077644.236398633175155No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA3193022.2256839108109285No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG3177472.214844835323425No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA3037182.1170561600731395No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG2305671.6071595613680572No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAA1808371.2605182597644735No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG1755461.2236375212407542No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT1732091.2073475409100167No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1515841.0566112017349214No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG1515521.056388146805275No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA1500951.0462321770398129No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT1407090.9808073779879078No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG1307610.9114651767340883No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA1188530.8284608610394275No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1109900.7736520825453802No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG1098840.765942746539477No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG1044710.7282116110964808No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG1030750.7184808397906574No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA865550.6033287323607116No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATT852820.5944553284407164No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG851410.593472492656962No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGA838240.584292388208703No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACT831740.5797615849502609No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA800000.5576373241159601No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAAT765820.5338122694431058No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATGG714870.4982977423634705No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT627480.4373828351703533No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGA623460.4345807076166706No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG607830.42368586839675504No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATT600570.41862530968040274No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT565530.39420079488412363No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT554370.386421754212706No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAA532590.371240078063649No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTC529260.3689189127020163No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGGAA525930.36659774734038364No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG524930.36590070068523867No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGATG492190.343079393195793No Hit
ATATGTGATGAATACGTGTTTGGAACTCTGAGGTGGA482710.3364713909050189No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG454760.31698893689371754No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT454430.3167589114975197No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGTGG450840.3142565140055493No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG444020.3095026558174607No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA441790.30794824177648755No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA423780.29539443151732697No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAAT421900.2940839838056544No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAA413080.287936032307276No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG393130.2740299515371343No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG356420.24844136882676313No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGA312900.2181058983948549No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAA312170.2175970543365991No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA308200.21482977911567364No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTG296080.20638157365531684No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGA294810.20549632440328278No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT294610.20535691507225376No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG292070.2035864165681856No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTGGAA290420.20243628958719642No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG272770.19013341612388804No Hit
GGCGGCGACTCTGGACGCGAGCCGGGCCCTTGGAATT267270.18629965952059083No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG258190.17997047589187468No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG252230.17581607782721076No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA249920.17420590005382594No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA246500.1718220004932302No Hit
CATATGTGATGAATACGTGTTTGGAACTCTGAGGTGG243960.17005150198916202No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT240720.1677930708264924No Hit
TTACATGATGAATACGTGTCTGGAACTCTGAGGTGGA239130.16668476664481194No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG238290.16609924745449017No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC237830.1657786059931235No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT234710.16360382042907123No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGA225720.15733737099931816No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGC224920.15677973367520218No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG223470.155769016025242No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTGGA219850.1532457071336173No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT208590.14539696179668515No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT199690.1391932465658951No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATG198720.1385171113104045No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTGGA198020.13802917865180303No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC194360.13547798789397253No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGACT192820.1344045360450493No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG190030.13245977587719487No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGG190030.13245977587719487No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC187200.13048713384313468No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT185940.12960885505765202No Hit
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGG185410.1292394203304252No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGA182530.12723192596360774No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG179750.1252941362623048No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGGAATT178090.12413703881476418No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTG176840.12326573049583299No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG174550.12166949365555106No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTG174340.12152311385797061No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC170540.1188743365684198No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG169950.11846307904188429No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAA163500.11396712811619936No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATTT163070.11366739805448703No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTA162580.11332584519346599RNA PCR Primer, Index 22 (97% over 37bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGA161510.11258000527246088No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA157530.109805759584984No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA155680.10851622327296584No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA151220.10540739519101937No Hit
GCAGTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT147610.10289105676594609No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGT147600.10288408629939465No Hit
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC147040.10249374017251348No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAA144380.10063959606982789No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)