FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16978869
Sequences flagged as poor quality0
Sequence length37
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA5297163.119854449669174No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA3520842.0736599122120563No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT2970031.7492507893193592No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG2830261.666930818536853No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT2601431.5321574128406314No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGT2576831.5176688152785678No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1775751.0458588260501922No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1673780.9858018222532963No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA1655100.9747999115842167No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG1510640.8897176837868294No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA1328780.7826080759560604No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG1270810.7484656369043191No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT1220130.7186167700569455No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA1157860.6819417712687459No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA1115500.6569931130277288No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG1007040.5931137109309225No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG928860.5470682411178271No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT914900.5388462564850461No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG895340.5273260545210638No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA871180.5130966026064516No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG824760.4857567368003134No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG794060.4676754382167622No Hit
CCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTG748940.4411012300053673No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT731820.43101810844997984No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG593420.3495050229788568No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC587620.346089012171541No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT558080.3286909157494531No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG512390.30178099613113213No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG491530.28949513657240655No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA468060.2756720721503888No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT462470.272379744492993No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG461680.2719144602623414No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT458930.2702947999657692No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT457980.2697352809542261No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA452580.26655485709913895No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG438950.2585272317019467No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC438290.25813851323076936No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG418690.24659475257156413No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA416490.24529902433430636No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGT411920.2426074434050937No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG398010.23441490714134142No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATT392810.23135227676236858No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATGGAA377550.22236463453484445No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG373850.2201854552267292No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGT373440.2199439786006948RNA PCR Primer, Index 15 (100% over 37bp)
GGACAGAAACTGTGTGCTGATTGTCACGTTCTGATTT370800.2183891047159855No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGA354280.20865936358894105No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT353720.20832954185582092No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT351560.20705737231378604No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG347840.20486641365805933No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT347580.20471328213911066No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG344780.20306417347350994No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG331870.19546060459032932No Hit
GGCTGGTCCGAGTGCAGTGGTGTTTACAACTGGAATT327080.1926394508373909No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG325000.19141439868580176No Hit
TGGCCAAGGATGAGAACTCTAATCTGATTTGGAATTC321550.18938246122282937No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG320220.18859913460666902No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG317570.18703837104815405No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG307420.1810603521353513No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA294230.1732918723856106No Hit
CCCAAATAAAGTATAGGCGATAGAAATTGAAACCTTG292020.17199025447454716No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTC290230.17093600286332383No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGA288050.16965205397367752No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG287100.16909253496213442No Hit
GGTTCGTGCTGAAGGCCTGTATCCTAGGCTACACACT285880.16837399475783693No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAAT284610.16762600618451087No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG273190.1608999986983821No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT266290.15683612377243736No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG264080.1555345058613739No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTC257050.15139406517595488No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCG250760.1476894603521589No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAA250560.14757166687604456No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAAT247350.1456810815844094No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG243900.14364914412143706No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCT243830.14360791640479703No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG241660.14232985718895647No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC238780.14063363113290997No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG238150.1402625816831498No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGA235240.13854868660568617No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA228460.13455548776541004No Hit
CCCCCACAACCGCGCTTGACTAGCTTGCTGTTTTGGA227790.134160879620427No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG221030.13017946012776233No Hit
CCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGG217070.12784714930069843No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACC212810.125338148259463No Hit
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCT212310.12504366456917712No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC211950.12483163631217133No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGG208210.12262889830883317No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC205260.12089144453614667No Hit
ATCAATGATGAAACTAGCCAAATCTGAGCTGGAATTC203650.11994320705342623No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT202160.11906564565637441No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG197040.1160501326678473No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGA196950.11599712560359586No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAAT194670.11465427997589239No Hit
TGAATCTGACAACAGAGGCTTACGACCCCTTATTTAC193450.11393573977159491No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT191150.11258111479628002No Hit
CTGGATGATGATAAGCAAATGCTGACTTGGAATTCTC189790.11178011915870249No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA187370.110354818097719No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG186910.11008389310265601No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA186810.11002499636459885RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGG185630.10933001485552425No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGA183570.10811674205154653No Hit
TTCTCACTACTGCACTTGACTAGTGGAATTCTCGGGT178440.10509533938921373No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG177630.10461827581095065No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAA177230.10438268885872197No Hit
TCTCAGTGATGTAATTCCAATAGATCCTTCTGACCTG176780.10411765353746472No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG175200.10318708507616144No Hit
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC175060.10310462964288139No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATT174030.10249799324089255No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATT172260.10145552097728064No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)