FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14207768
Sequences flagged as poor quality0
Sequence length37
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT3603272.5361267160330883No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA3209782.2591725878406796No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT2066501.4544860248281082No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA2009421.4143108192645037No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG1681651.1836130770153341No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1509311.0623132359706324No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1452851.0225744114064925No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA1340640.9435964889066318No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA1329700.9358964757870483No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1316880.9268732428626368No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGG1199200.8440453138029843No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA1187320.8356836907809868No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGA1174810.8268786483563076No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA1041720.7332045399389968No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG1006090.7081267092762213No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG985050.6933179089072964No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA978720.688862599670828No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG948470.6675714299388897No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGG904040.636299804445005No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT819020.5764593002926286No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT812870.5721306823140694No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG790030.5560549693660538No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT788770.5551681305606905No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG778410.5478763448277026No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGA643420.4528649397991296No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG633700.4460236118720407No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAA607460.42755484183018755No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG595420.41908060435671524No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG591000.4159696301347263No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG581130.4090227261593799No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG565410.3979583563019892No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG560250.39432654024192965No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT544670.38336070802957933No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA472950.3328812801560386No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA457760.3221899456691579No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC446010.31391982188898354No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAA442380.31136488152115094No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACT423430.29802710742461447No Hit
ATTGGTCGTGGTTGTAGCCCGTGCGAGAATACCATGG421430.29661942678118053No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT406590.28617443640690077RNA PCR Primer, Index 16 (100% over 37bp)
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG392370.27616582703208553No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT390370.27475814638865165No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTG388600.2735123490192126No Hit
GGTAAAATGGCTGAGTGAAGCATTGGACTGTACCACC387110.2724636269398543No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTG380420.2677549351875678No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT361060.2541285865591274No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG317820.22369453104808582No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG317650.22357487819339392No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT309620.2179230404100067No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG299520.21081425316066535No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT295830.20821708237352973No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC292900.20615483023089903No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT292840.20611259981159602No Hit
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC291750.20534541386092453No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG288530.20307904802499588No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG287060.20204440275207197No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA277600.19538607330862948No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA274500.1932041683113069No Hit
GAGAAAGCTCACAAGAACTGCTAACTTGGAATTCTCG272500.19179648766787294No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA265600.1869399894480259No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG259320.18251987222764338No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG258980.18228056651825958No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT252130.17745926031449838No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC248920.17519993288178692No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT240790.169477711066228No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCATGGAAT238430.16781664790697595No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT228280.16067266864154875No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG223660.15742092635521637No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGA223110.15703381417827206No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG204350.1438297697428618No Hit
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC204340.14382273133964463No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT202460.14249951153481674No Hit
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGT202330.14240801229299352No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT201840.14206313053535222No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG201480.1418097480195341No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAAT201010.14147894306832712No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGT198270.13955042058682265No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG196180.1380793943144342No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG196030.13797381826617666No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG195180.13737555399271723No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT188080.13237828770852678No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAA184550.1298937313728659No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG181950.1280637465364018No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG181860.12800040090744724No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG181470.12772590318197763No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG177810.12514984760449355No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTC175820.12374920536427678No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC174810.12303832663934265No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG174450.12278494412352454No Hit
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTG173430.12206702699537324No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGA169180.11907570562807612No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT167360.11779471624255126No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG166280.11703456869509693No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG164720.11593657779321848No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC161790.11387432565058775No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGA161410.11360686632833532No Hit
GGACAGAAACTGTGTGCTGATTGTCACGTTCTGATTT161350.11356463590903229No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTG160950.11328309978034551No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT158310.11142496133101272No Hit
TGTCGCTGATGCCATCACCGCAGCGCTCTGACCTGGA156320.11002431909079596No Hit
GTGGACAGAAGGGATTTCTGAAATTCTATTCTGAGGC150190.10570977791867096No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA150190.10570977791867096No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGT148980.10485813112939345No Hit
TGAGAACTGAATTCCATGGGTTGTGGAATTCTCGGGT148870.10478070869400456No Hit
TTGGTCGTGGTTGTAGCCCGTGCGAGAATACCATGGA148670.10463994062966117No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA142980.10063508919909164No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)