FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14831926
Sequences flagged as poor quality0
Sequence length37
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG7510625.063819762854804No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT4539833.0608499530000355No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG3952332.664744956251804No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA3658022.466314893965895No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA2418681.6307255038893802No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG2208081.488734504203972No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG2116911.427265750921357No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1978841.3341760200259898No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG1924691.2976669381980466No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG1788141.2056020236346918No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT1620561.09261602303032No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1377860.9289825205438593No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1358320.9158082369073308No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA1338640.9025395622928539No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG1266770.8540832795417129No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT1237730.8345038938301068No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTC1133820.7644455615541771No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT1067830.7199536998768737No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA1054340.7108584549302632No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG872560.5882985122768277No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT806330.5436448374944697No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT805910.5433616645606242No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG791560.5336865893209014No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATT693860.46781517113825943No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT630270.42494144051150207No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA609170.4107153716921187No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT556850.3754401147902167No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG524060.3533323993121325No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG522810.3524896227233065No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGT521070.35131647771166064No Hit
ATATGTGATGAATACGTGTTTGGAACTCTGAGGTGGA495170.33385414679118547No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTG481430.3245903465268098No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT440500.2969944699022905No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG439030.2960033646338311No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTG438380.2955651208076416No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG438030.2953291433627703No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC407350.27464403476662436No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC404430.2726753086551268No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA401210.2705043161623109No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC400110.26976267276414406No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGC398310.26854907447623455No Hit
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGC363710.24522101849753028No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG351220.23679999482198064No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG350580.23636849320850173No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC347540.23431886054447681No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG332720.22432690130735547No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTG332430.22413137713874787No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG329640.2222502997924882No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG328010.22115131912065905No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTG324620.2188657090117629No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA320820.21630366818173175No Hit
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTG311060.20972326857617818No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG302690.20408003653739912No Hit
ACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGT287140.1935958957724034No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGTGG286350.19306326096826537No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC284530.19183617825493465No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG277190.18688739412534824No Hit
TACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTG271220.18286229313711516No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT261910.17658529310353893No Hit
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGT261880.17656506646540712No Hit
TGATGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG260170.17541214809189312No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA258290.17414461210229878No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGGAA254110.17132636718926458No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAA251750.16973520498956102No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATT247420.16681582688586769No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAG234280.15795655938412853RNA PCR Primer, Index 3 (97% over 37bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGC234070.15781497291720575No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCC231990.15641259267339927No Hit
TACCCTGTAGAACCGAATTTGTGATGGAATTCTCGGG230460.1553810341286762No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTG230270.15525293208717464No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG228850.1542955378822683No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA223200.15048618770077468No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG221410.1492793316255758No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT220060.1483691329096437No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG216800.14617117156598544No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG214700.14475530689675772No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT212600.14333944222753No Hit
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGG212510.14327876231313452No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG212210.14307649593181626No Hit
TTACATGATGAATACGTGTCTGGAACTCTGAGGTGGA206880.1394828965570621No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG204140.13763553027435546No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCT202040.13621966560512774No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG201010.1355252176959351No Hit
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGG195610.13188442283220667No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG194540.1311630060721716No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA193230.13027977620708195RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG192550.12982130574276057No Hit
CCCAGTGTTTAGACTATCTGTTCTGGAATTCTCGGGT192020.12946396846909836No Hit
CATATGTGATGAATACGTGTTTGGAACTCTGAGGTGG190220.12825037018118887No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTC190170.12821665911763583No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCT188810.1272997181889931No Hit
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGT184520.12440730893614221No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA180580.12175087712816257RNA PCR Primer, Index 1 (100% over 32bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG179730.1211777890477609No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA177200.11947200923197701No Hit
AGGCAAGATGCTGGCATAGCTGTGGAATTCTCGGGTG175910.11860226379230855No Hit
TACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGG175180.11811008226443416No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG164360.11081500811155612No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG163130.10998571594815132No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGA161940.10918339263558893No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG161580.10894067297800704No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG159300.10740344847998838No Hit
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTG158660.10697194686650943No Hit
AGCTACATCTGGCTACTGGGTCTTGGAATTCTCGGGT158570.10691126695211398No Hit
TGAGGTAGTAGGTTGTATAGTTTTGGAATTCTCGGGT154360.10407279540094792No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGT151340.10203664716234426No Hit
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGT151260.10198270946065939No Hit
GGACAGAAACTGTGTGCTGATTGTCACGTTCTGATTT151140.1019018029081321No Hit
GGCGGCGACTCTGGACGCGAGCCGGGCCCTTGGAATT149910.1010725107447273No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG148580.10017579645421641No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)