FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13218489
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA3153852.3859383625465815No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGT3080102.3301452987554025No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG1929581.4597583732906234No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA1723771.3040597907975715No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1565481.1843108542890188No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1300100.9835466065750783No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1051770.7956809586935389No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1012810.7662070906894124No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATT999080.755820124372763No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT966420.7311123079196117No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG951590.7198931738718397No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG951440.7197796964539593No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA910330.6886793187935474No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT815580.6169994164991173No Hit
CCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTG801620.6064384514750514No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT775860.5869505962443968No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG755400.571472276445515No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC689270.5214438654826584No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA687350.5199913545337898No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT684700.5179865868179033No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG682100.5160196449079769No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG642160.485804391107032No Hit
GTAGAATTCTCGCCTCCCACGCGGGAGACCCGGGTTT636370.4814241627768499No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC631050.477399497022693No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG615610.46571888814220747No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA588060.4448768690581806No Hit
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC579580.43846161236734393No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT546140.4131637133412147No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG538160.4071267147099793No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG507620.38402271242953717No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT497760.3765634634942012No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG492170.3723345383878596No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA467940.35400415281958475No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG422560.31967345133017855No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT418670.31673060362648103No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC417960.3161934771818473No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG398510.30147923866336007No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA393830.29793874322549274No Hit
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCT374690.28345902470395823No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG372770.28200651375508956No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA362430.2741841370825364No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC362120.2739496170855837No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG355630.2690398274719599No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGA355490.2689339152152716No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCC351280.26574898235342936RNA PCR Primer, Index 18 (100% over 37bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA350000.26478064172085025No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG349770.2646066430134337No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT348940.26397873463449567No Hit
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTC331120.25049761739030835RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG321420.24315941103404481No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG308560.23343061374110158No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATGGAA304040.23001116088230658No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG303310.2294589041152888No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT298780.22603188609530178No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG288000.21787664233029963No Hit
AGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCT261400.19775331355951503No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGT261370.19773061807593895No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG258770.1957636761660126No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC257430.19474994456628136No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG253030.19142127364179068No Hit
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCC250000.1891290298006073No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGA249390.18866755496789384No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC247400.187162087890681No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATGGAATT247010.18686704660419207No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT246570.18653417951174298No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGT245280.18555827371797184No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG241460.18266838214261857No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG236760.17911275638236715No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA234330.17727442221270526No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACTGGAA228740.17304549710636366No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGA225700.17074568810398827No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA218200.16507181720997005No Hit
TCGAGAGGGGCTGTGCTCGCAAGGTTTCTTTTGGAAT216170.16353608948798914No Hit
CTGGATGATGATAAGCAAATGCTGACTTGGAATTCTC215430.16297626755977934No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT210940.15957951018456043No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT202280.15302808059226738No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAAT198770.15037270901386687No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG197450.14937410773651966No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG183920.13913844464371078No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT180780.13676298402941517RNA PCR Primer, Index 1 (100% over 25bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC180050.13621072726239739No Hit
CCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT178350.13492464985975325RNA PCR Primer, Index 1 (100% over 30bp)
TCTCAGTGATGTAATTCCAATAGATCCTTCTGACCTG177380.13419082922412692No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCATGGAA176900.13382770148690973No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGG175930.13309388085128337No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAA174800.13223901763658463No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT171540.1297727750879847No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAA170970.12934156090003934No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG170080.12866826155394917No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCG161740.1223589171198009No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGC159540.12069458165755557No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATGG158700.12005910811742553No Hit
TTCTCACTACTGCACTTGACTAGTGGAATTCTCGGGT158650.12002128231146542No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT158500.11990780489358503No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAA152030.11501314560234531No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT148460.11231238305679264No Hit
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT145430.11002013921560928RNA PCR Primer, Index 1 (100% over 30bp)
TGAGAACTGAATTCCATGGGTTGTGGAATTCTCGGGT142770.10800780633853083No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAAT141230.10684277151495908No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG141070.1067217289358867No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGA139120.10524652250344195No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGG137930.10434626832159107No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTC136020.10290132253391443No Hit
CCCCCCACTGCTAAATTTGACTGGCTTGGAATTCTCG132300.10008708257048138No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)