FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17168626
Sequences flagged as poor quality0
Sequence length37
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG3885872.26335526209261No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG3803992.21566361804375No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG2772551.6148933525606535No Hit
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC2390581.3924119495642808No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1794131.045004999235233No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1401300.8161981046124483No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT1194590.6957982543273993No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA1186020.6908065910457831No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGT1152800.6714573431793552No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1104710.6434469479386411No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT1006950.5865058741450829No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA979760.5706688467673534No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG968430.5640695999784724No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT951970.5544823447141314No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG951340.5541153963048644No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA868350.5057772241063437No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG856700.49899159082386674No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT786770.45826031739523015No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG726120.4229342522808756No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG723110.4211810543254888No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA719660.4191715749414076No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG679660.39587326324191585No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG635640.37023347121662503No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC616870.35930073845163846No Hit
CTGGATGATGATAAGCAAATGCTGACTTGGAATTCTC594320.34616631523104996No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC565590.32943230285288994No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA560400.3264093469098808No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC547840.3190936770362404No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG547630.3189713608998181No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC533160.3105431966425269No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA531060.3093200352783036No Hit
GGACAGAAACTGTGTGCTGATTGTCACGTTCTGATTT513710.29921439257864896No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGA458490.2670510732775005RNA PCR Primer, Index 19 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT445160.2592869109036448No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG433870.2527109624264633No Hit
TTCTGTGATGAAAATGTTTCTGAAGTGGAATTCTCGG429920.25041025414613843No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG417220.24301304018154976No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT407680.23745639284122097No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTC406310.23665842566551334No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG402820.2346256479697327No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG400510.23328017046908706No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG398400.23205118452693882No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC397190.2313464105980292RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT396890.23117167326028304No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTC392930.22886514040203332No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT388630.22636057189433798No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG379120.22082139828778377No Hit
TTCCCTTTGTCATCCTTCGCCTTGGAATTCTCGGGTG377880.22009915062509955No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA372750.21711114214963972No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT372700.21708201926001533No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA370320.21569576971389556No Hit
GTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTC348170.20279432961030197No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT329550.1919489655141885No Hit
TCAGTGCACTACAGAACTTTGTCTTGGAATTCTCGGG328950.19159949083869612No Hit
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCT315410.18371301232841813No Hit
CGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCT313240.1824490789187207No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT308310.1795775620017583No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA306480.17851166424150658No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG306450.17849419050773194No Hit
GTGAATGATGAACATGAACTTTCTGACCTGGAATTCT303720.17690408073424163No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG301480.17559937527907007No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG298290.1737413349210356No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG296520.1727103846283331No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCATGGAAT296310.17258806849191077No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA289940.1688778123537667No Hit
ATTAATGATGAGATATAACCTTGACTGAAGTGGAATT289440.16858658345752306No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG287260.16731682546990073No Hit
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGT284610.1657733123198094No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG277720.16176017812957194No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC274600.15994290981701156No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT274500.15988466403776283No Hit
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGA274440.1598497165702136No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG268190.15620935536716798No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA258550.15059446224759046No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG250430.1458649049725936No Hit
TAGACGGGCTCACATCACCCCATAAACACCATGGAAT247090.14391949594568604No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCG245550.1430225109452556No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA245320.14288854565298353No Hit
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCT245190.1428128261399602No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGT242060.14098973324947495No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG236290.13762895178682324No Hit
TGTCGCTGATGCCATCACCGCAGCGCTCTGACCTGGA234590.13663877353959486No Hit
ATCAATGATGAAACTAGCCAAATCTGAGCTGGAATTC228230.13293434197937565No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA224700.1308782659718955No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGGAATTCT224690.1308724413939706No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT220330.12833292541872598RNA PCR Primer, Index 1 (100% over 25bp)
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGT216670.1262011298982225No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT215260.1253798644108154No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA213720.12448287941038497RNA PCR Primer, Index 1 (100% over 22bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTG211120.12296848914991801No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGT205960.11996300694068354No Hit
TACTGTGATGAGCTCAGATGGGGAGACTGAGGTGGAA201220.11720215700429376No Hit
GTGTATGATGACCTCATGTAGCCTGAACTGGAATTCT201020.1170856654457963No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG200840.11698082304314861No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGT199310.11608966262064302No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG198570.11565864385420244No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA198010.11533246749040954No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATG197790.11520432677606234No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATGG192630.1121988445668279No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTC192050.11186101904718526No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA190030.11068445430636091RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG189640.11045729576729087No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGA187410.10915841489004419No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG186860.1088380631041762No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC181980.10599566907683819RNA PCR Primer, Index 1 (100% over 24bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG181150.10551222910907372No Hit
AAACCTGATGTTACATTGTAGTGTGCTGATGTGGAAT179300.10443468219297222No Hit
CATTGGTCGTGGTTGTAGTCCGTGCGAGAATGGAATT179160.104353138102024No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT176100.10257081725701288No Hit
CCCTGGCGGATGGAATTCTCGGGTGCCAAGGAACTCC174950.10190099079565249RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA173000.10076519810030225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)