FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16095097
Sequences flagged as poor quality0
Sequence length37
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT3187901.980665292045149No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG2489061.5464709532350132No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA1882781.1697848108650728No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1470650.9137254655874394No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG1339100.8319925005733112No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG1260670.7832633751756823No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1256150.7804550665336158No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG1233840.7665937024175747No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG1180750.7336085020177263No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1009760.627371180179902No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG961610.5974552374552325No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG942800.5857684486151279No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA924250.5742431996526645No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG867570.5390275063269268No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA774250.48104711639824227No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG765710.47574115272495715No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG757950.4709198086845951No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT747850.46464460574546396No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACT707630.4396556292888449No Hit
TCACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA644460.40040765209429924No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG555750.345291488457634No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG554700.3446391158748531No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT539250.3350399192996476No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCATG494090.30698168516784957No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA475480.2954191577720843No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAT460660.28621138474654734No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG459540.28551552065824765No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTC436370.2711198323315479No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGG422590.262558218816575No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT421440.26184371551162444No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT420340.26116027756775867RNA PCR Primer, Index 1 (100% over 25bp)
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA411490.2556617086557478No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG406860.2527850562192946No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT406470.25254274640283314No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG392970.24415509890993511No Hit
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGG391090.24298704133314636No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA388520.24139028177338728No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG378010.23486034287336074No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG372920.23169788911492736No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCCTTG366200.22752270458512922No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT365180.2268889712189992No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG356230.22132827158481866No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGG350830.21797321258765945No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG345870.21489152876804657No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG339040.21064800044386187No Hit
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTC335910.20870330883995292No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCA334640.20791424866839883No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCCCTG329840.20493197400425733No Hit
AGCTACATCTGGCTACTGGGTCTTGGAATTCTCGGGT312910.19441324274094154No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGTGGAATTC306710.19056113796642543No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGTGG305260.18966024249496602No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGA299970.1863735272921934No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT298710.18559068019285624No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA296290.18408711671635156No Hit
CACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG294470.18295633757286459No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGT289980.1801666681474489No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA288800.17943352562584744No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT280340.17417726653029802No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGATG280150.17405921815817574No Hit
AGGCAAGATGCTGGCATAGCTGTGGAATTCTCGGGTG271900.16893343357918253No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA271070.16841774858517472No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG264810.16452836537735685No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATGGAAT260570.16189402275736517No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA259370.1611484540913298No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGTGGAATT257650.16007980567001243No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA253800.15768777286648228No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGGAATTCT252350.15678687739502284No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA250910.1558921949957804No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGTGG244390.1518412719103215No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGT244340.15181020654923671RNA PCR Primer, Index 23 (100% over 37bp)
CCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGG242670.15077262348900414No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC242420.1506172966835801No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGA239130.1485731959241998No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAA236860.14716282853094953No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTC235070.1460506886041134No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG222400.13817872610522322No Hit
TTCTGTGATGAAAATGTTTCTGAAGTGGAATTCTCGG221380.13754499273909315No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGG217640.13522130372994956No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCACT214610.13333874284821023No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG213460.13262423954325966No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG205390.12761029026417176No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTG200690.12469014632219987No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC197720.1228448638737623No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG195630.12154633178041735No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGG194440.12080697618659893No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG193450.12019188203711975No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA192930.11986880228183774No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTG190580.1184087303108518No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCT189030.11744570411722279No Hit
CCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTC188910.11737114725061924No Hit
CCCCGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACT187830.1167001354511874RNA PCR Primer, Index 1 (100% over 25bp)
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT184930.11489834450826857No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCTGGA182540.11341342024841479No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT180880.11238205026039919No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG180450.11211488815506983No Hit
CCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCG178270.11076043841177223No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG176200.1094743324628612No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG169530.1053302132941479No Hit
TCGAGGCCAGCCTGGTCCACATGGGTCGGAAAAAAGG167770.10423671258396269No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT167670.10417458186179306No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA167670.10417458186179306No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT167430.104025468128586No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGGAATTCTC162800.10114881569213283No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)