FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19496128
Sequences flagged as poor quality0
Sequence length37
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG6513363.3408479878671296No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG4452292.283679097716224No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG3453151.771197850157734No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG2445441.2543208579672847No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG2260751.1595892271532071No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT2239091.1484793288185224No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT2234871.1463147964559937No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT2169501.1127850617312318No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1862190.9551588910372357No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG1797970.9222190170273811No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1716730.8805492044369015No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA1628950.8355248796068635No Hit
TCACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA1623120.8325345422434649No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA1611870.8267641656845913No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG1327030.6806633604375186No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG1220940.6262474271814383No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG1191250.6110187622896198No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG1167390.5987804347611998No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1034600.5306694744720593No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG1032150.5294128146881268No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG1028130.5273508667977559No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG890920.4569727896739291No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTC837690.42966993240914303No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT801260.41098417080560823No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG715600.3670472413804423No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA684560.35112613130155895No Hit
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGG663310.3402265311347976No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG659470.33825690926936874No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG628510.3223768329793485No Hit
AGCTACATCTGGCTACTGGGTCTTGGAATTCTCGGGT625390.32077651521368755No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGA623800.31996096866003343No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG617900.3169347267313797No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG589090.30215743351705526No Hit
GTAGAATTCTCGCCTCCCACGCGGGAGACCCGGGTTT563910.2892420484723941No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG557310.2858567608911883No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT535620.27473147488567984No Hit
CCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGG531140.2724335827093462No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCCTTG525450.26951505447645807No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT521940.26771469699008954No Hit
AGGCAAGATGCTGGCATAGCTGTGGAATTCTCGGGTG506300.25969259126735317No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT476240.24427414510204282No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA466760.23941164112176533No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG457050.23443116499850636No Hit
CCCCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGT456870.2343388389735644No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAT445110.22830687201068847No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGTGG442760.22710150446283486No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG425300.218145880043463No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC413550.21211904230419498No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA409990.21029303869978694No Hit
CCCCGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC408540.20954930127664323RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG389450.19975761340918566No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGG385090.1975212719161466No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT375170.19243308209712207No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT368680.18910421597560295No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG365760.18760648268209976No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG355360.18227209012989656No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG354340.18174890932189203No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC353980.18156425727200806No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA351090.18008191164932852RNA PCR Primer, Index 1 (100% over 22bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA346890.17792763773401568No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG346180.17756346285785568No Hit
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGG344800.17685562999996718No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA336160.1724239808027522No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG332200.1703928082540287No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG328920.16871042291064153No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG322180.16525332619892524No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA317990.1631041815072203No Hit
CCCCCCTCGCGGCCTGGAATTCTCGGGTGCCAAGGAA316620.16240147787293968RNA PCR Primer, Index 1 (100% over 23bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG307970.15796469945211683No Hit
CCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCG302880.15535392463570202No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT301180.1544819566223611No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT297870.1527841836081503No Hit
GCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC296660.1521635475515959RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTA294660.15113770283001834RNA PCR Primer, Index 22 (97% over 37bp)
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT294380.15099408456899752RNA PCR Primer, Index 1 (100% over 25bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGC294160.15088124164962397No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG286250.1468240257757848No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCA286180.14678812121052962No Hit
CCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTC286150.14677273353970594No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATGGAAT284510.14593154086801235No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG283830.145582753662676No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG275300.1412075259251478No Hit
GAGAAAGCTCACAAGAACTGCTAACTTGGAATTCTCG268020.13747345113860557No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTG267880.13740164200809513No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC264400.13561667219255022No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCT260170.13344701060641373No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCA258310.13249297501534663No Hit
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTG257500.13207750790310774No Hit
GCTCCGGGACTGGAATTCTCGGGTGCCAAGGAACTCC256350.13148764718820066RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACT246870.12662514320792312No Hit
TCGAGGAGCTCACAGTCTAGTATGGAATTCTCGGGTG246350.12635842358031296No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCG244440.12537874187120643No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT243100.12469142590774947No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGA240050.12312701270734373No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG239100.1226397364645944No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA234930.12050085022010525No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG232110.11905440916268092No Hit
CCCCGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACT229660.11779774937874844RNA PCR Primer, Index 1 (100% over 25bp)
TTCTGTGATGAAAATGTTTCTGAAGTGGAATTCTCGG229420.11767464801215914No Hit
CCCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGTG228090.11699246127231007No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA224690.11524852524562826No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCAT223870.11482792890978148No Hit
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTC223170.11446888325722933No Hit
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC218980.1123197385655244RNA PCR Primer, Index 1 (100% over 27bp)
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGT218250.11194530524214859No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTC216560.11107846645241558No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGGAATTCT209600.10750852682132575No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG207930.10665194647880852No Hit
CCCGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTC207690.1065288451122192RNA PCR Primer, Index 1 (100% over 26bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT207410.10638522685119836No Hit
AGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCT207370.10636470995676679No Hit
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTG206880.10611337799998029No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC206350.10584152914876226No Hit
TAGTTCACTGATGAGAGCATTGTTCTGAGCCAGTGGA202720.10397962097909903No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG202590.10391294107219648No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCCTGG201420.10331282191007364No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCCCTG200400.10278964110206909No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT196700.10089182836715066No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)