FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15805163
Sequences flagged as poor quality0
Sequence length37
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT2865581.8130657684454123No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG2711811.715774775622371No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG2389371.5117654908082885No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1770561.120241531200912No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG1769841.1197859838585658No Hit
CCCCGGGGAGCCCGGCGGGCTGGAATTCTCGGGTGCC1677091.0611026282993727No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA1480990.937029247974222No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1201880.7604350553043964No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1156820.7319253841292241No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1102440.6975189056892359No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT1094170.6922864382986749No Hit
CCCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1054210.6670035607984556RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA960280.6075736137615284No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG947260.5993357993207662No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC834350.527897118175877RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT710430.44949235892094247No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA705150.44615167841040293No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT705080.44610738908545267No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG569760.360489796910035No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA550720.3484431005235441No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA549060.347392810817579No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG547400.34634252111161395No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG518730.32820287902124134No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA507510.3211039329363449No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT504510.319205819009902No Hit
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTC503050.3182820702323665RNA PCR Primer, Index 1 (100% over 26bp)
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT490150.310120180348662RNA PCR Primer, Index 1 (100% over 25bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG468770.29659295509954564No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG428150.27089249253550884No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGT418230.2646160624854043No Hit
CCCGGTCAGCCCCTCTCCGGCCCCGTGGAATTCTCGG413990.26193339480269834No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA391490.24769754035437658No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT383360.24255365161371636No Hit
CCGGTCAGCCCCTCTCCGGCCCCGTGGAATTCTCGGG382960.2423005697568573No Hit
CCCCGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACT379820.24031387718051372RNA PCR Primer, Index 1 (100% over 25bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCT376170.23800450523667488No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG371170.2348409820259367No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG360970.22838739467603086No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG360720.22822921851549396No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTT358760.2269891174168846RNA PCR Primer, Index 21 (100% over 37bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTC354720.22443299066260816No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC353650.2237559966955102No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG344680.21808063605544595No Hit
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCA344260.21781490010574392No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG339060.21452483596657623No Hit
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCT337860.21376559039599907No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT336020.21260141385444745No Hit
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC334900.21189278465524206No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG323560.20471791401328793No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG317500.20088372388187328No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG315110.19937155978714044No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC302320.19127926741407222No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC299540.18952034850890181RNA PCR Primer, Index 1 (100% over 24bp)
CCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTG296830.18780571892868173No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT296280.1874577313755005No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC288430.18249099993464163No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG286660.18137111271804032No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG281720.17824555178583099No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG281600.17816962722877328No Hit
CTGGATGATGATAAGCAAATGCTGACTTGGAATTCTC275660.17441136165441634No Hit
CCTCCCCGGAGGGGGCGGGCTGGAATTCTCGGGTGCC272800.17260182637787413No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA269960.17080494519417486RNA PCR Primer, Index 1 (100% over 22bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG269640.17060247970868758No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG263600.16678094367011592No Hit
CGGCTCCGGGACTGGAATTCTCGGGTGCCAAGGAACT262210.16590148421753068RNA PCR Primer, Index 1 (100% over 25bp)
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA261670.16555982371077096RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG261570.1654965532465562No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG260040.16452851514407033No Hit
CCCGCCGGGTCCTGGAATTCTCGGGTGCCAAGGAACT256100.16203565885400867RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG251810.1593213559391953No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC242740.15358272483491628No Hit
CCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG233000.14742018162039833RNA PCR Primer, Index 1 (100% over 29bp)
GCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG232960.14739487343471244RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG223070.14113742452387237No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGT220280.13937217857228046No Hit
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGC219310.13875845506939724No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT217780.13779041696691138No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAA212360.13436115780647123No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA210650.13327923286839877No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG208290.13178604991293036No Hit
CCGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAGGAA203400.12869212421282844RNA PCR Primer, Index 1 (100% over 23bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG200370.1267750291471211No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG199890.12647133091889023No Hit
CCGCGAGGGGGGCTGGAATTCTCGGGTGCCAAGGAAC199310.1261043622264446RNA PCR Primer, Index 1 (100% over 24bp)
GTGGACAGAAGGGATTTCTGAAATTCTATTCTGAGGC194260.12290920378359907No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT194150.12283960627296282No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC193780.12260550555536821No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT193360.1223397696056662No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG189810.12009366812604211No Hit
GGACAGAAACTGTGTGCTGATTGTCACGTTCTGATTT188530.11928380618409314No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTG187440.11859415812415222No Hit
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTC185820.11756917660387305RNA PCR Primer, Index 1 (100% over 26bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC181400.11477262208558052No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT178820.11314024410883962No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG177920.11257080993090675No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATT177820.112507539466692No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG176220.11149521203925579No Hit
CCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA175680.11115355153249605RNA PCR Primer, Index 1 (100% over 28bp)
CCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAACTC172580.10919216714183841RNA PCR Primer, Index 1 (100% over 26bp)
CCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAAC169570.10728772616897403RNA PCR Primer, Index 1 (100% over 24bp)
GCCGGGCGCGTGGAATTCTCGGGTGCCAAGGAACTCC166910.10560473182086132RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC165460.10468731008974724No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTG164400.10401664316907076No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGC164040.10378886949789762No Hit
GCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC161290.10204893173199163RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT161170.1019730071749339No Hit
CCCTGGCGGAGCTGGAATTCTCGGGTGCCAAGGAACT160640.10163767371459567RNA PCR Primer, Index 1 (100% over 25bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACTGGAA159160.10070127084421718No Hit
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC158680.1003975726159863RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)