FastQCFastQC Report
Sat 1 Apr 2023
EGAF00004170958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34825038
Sequences flagged as poor quality0
Sequence length37
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGGGGAGCCCGGCGGGCTGGAATTCTCGGGTGCC8834422.5368012520187344No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG7278112.0899072672942958No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG5252161.5081562868646403No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT4682641.3446187768696765RNA PCR Primer, Index 1 (100% over 25bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT4126571.1849434306432056No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG3416120.9809379102472192No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA3367630.9670140202000641No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG3233410.9284727844374498No Hit
GCCCCGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAG3071830.8820751322654694No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG2805000.80545497179357No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGT2582750.7416359459535982No Hit
CCGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAGGAA2577120.7400192930155597RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT2400590.6893287524912391No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG2352270.67545367789692No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG2272370.6525104150640123No Hit
ACGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAA2061380.5919246951001174No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG1947130.5591178392971172No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT1827570.5247862184672993No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC1802830.5176821343310523RNA PCR Primer, Index 1 (100% over 27bp)
CCCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1799400.5166972107826558RNA PCR Primer, Index 1 (100% over 28bp)
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCA1727690.49610570417755173No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1650090.4738228857065425No Hit
CCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA1616790.46426079994514297RNA PCR Primer, Index 1 (100% over 28bp)
CGGCTCCGGGACTGGAATTCTCGGGTGCCAAGGAACT1359610.3904116342959913RNA PCR Primer, Index 1 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT1343600.38581436723773277No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG1290440.3705494879862012No Hit
CCGCGAGGGGGGCTGGAATTCTCGGGTGCCAAGGAAC1272900.3655128818524189RNA PCR Primer, Index 1 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA1231650.3536679557966312No Hit
CCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAAC1209530.3473162039335032RNA PCR Primer, Index 1 (100% over 24bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT1142530.32807717252167823No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG1088670.31261128846435143No Hit
CGGCCGGGCGCGACTGGAATTCTCGGGTGCCAAGGAA1043900.2997555953851364RNA PCR Primer, Index 1 (100% over 23bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG1001690.28763500559568667No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG954900.27419927007689127No Hit
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAA954840.2741820410935374RNA PCR Primer, Index 1 (100% over 23bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT946480.27178146941289766No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG943580.2709487352174605No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA914330.26254960583244735RNA PCR Primer, Index 1 (100% over 23bp)
CCCTGGCGGAGCTGGAATTCTCGGGTGCCAAGGAACT911320.26168528516752804RNA PCR Primer, Index 1 (100% over 25bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG906860.26040459740489014No Hit
CCCGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCA901780.2589458768142622RNA PCR Primer, Index 1 (100% over 28bp)
CCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA880800.2529214756348579RNA PCR Primer, Index 1 (100% over 28bp)
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC874800.25119857729947054RNA PCR Primer, Index 1 (100% over 26bp)
CCCCGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACT861390.2473478995198799RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTC835560.23993082218603753RNA PCR Primer, Index 1 (100% over 26bp)
GCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC818210.23494877449954255RNA PCR Primer, Index 1 (100% over 24bp)
CCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG763380.2192043552113281RNA PCR Primer, Index 1 (100% over 29bp)
CCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTG762910.2190693948417228No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG752620.21611462419653354No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA751990.21593371987131788RNA PCR Primer, Index 22 (100% over 37bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG712160.20449654642157172No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT704070.20217350516602453No Hit
CCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAACTC702760.20179733902946495RNA PCR Primer, Index 1 (100% over 26bp)
CCCGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAGGA702210.20163940668205446RNA PCR Primer, Index 1 (100% over 22bp)
CGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAG693040.1990062437261375No Hit
CCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG682110.19586769725850692RNA PCR Primer, Index 1 (100% over 29bp)
CCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCC665640.19113834132786875RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG665450.19108378288058148No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGA623680.17908953896906013No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG613510.17616922629057863No Hit
CCCTGGCGGATGGAATTCTCGGGTGCCAAGGAACTCC612920.17599980795426554RNA PCR Primer, Index 1 (100% over 27bp)
GCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG611380.17555759738151613RNA PCR Primer, Index 1 (100% over 29bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG611320.17554036839816226No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG596460.171273323520853No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC588610.1690191981987213No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG577970.16596392515063446No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG571680.1641577533957034No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA554770.1593020515871368No Hit
GCCGGCGGGAGCCCCGGGGATGGAATTCTCGGGTGCC553870.15904361683682872No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT529000.1519022032366483No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT526130.151078083532888No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG525470.15088856471599543No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG518320.14883544419965888No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC505480.14514844176193004RNA PCR Primer, Index 1 (100% over 24bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGC496560.1425870662366542No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG490310.14079238047062576No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT484750.13919582801316685No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA481970.13839755178443738RNA PCR Primer, Index 1 (100% over 22bp)
GCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC479420.1376653199918978RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG473470.1359567791426387No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATT467020.13410466343209734No Hit
GCCCCGCCGGGGTCGGCCTGGAATTCTCGGGTGCCAA465250.13359640842315806No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG450850.1294614524182285No Hit
CGGCCGGGCGCGATGGAATTCTCGGGTGCCAAGGAAC444670.1276868671327796RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAGGAAC432640.12423245597032802RNA PCR Primer, Index 1 (100% over 24bp)
CCGAGGGCGCTGGAATTCTCGGGTGCCAAGGAACTCC428940.12317000199683918RNA PCR Primer, Index 1 (100% over 27bp)
CCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCA426520.12247509966823295RNA PCR Primer, Index 1 (100% over 28bp)
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA424590.12192090070368337No Hit
CCCCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG424150.12179455482575496RNA PCR Primer, Index 1 (100% over 29bp)
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA423720.1216710804450522RNA PCR Primer, Index 1 (100% over 28bp)
CCCTGGCGGAGCGTGGAATTCTCGGGTGCCAAGGAAC414750.11909534743364818RNA PCR Primer, Index 1 (100% over 24bp)
CCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC409240.11751315246231749RNA PCR Primer, Index 1 (100% over 27bp)
CGACGGGCGCTGGAATTCTCGGGTGCCAAGGAACTCC404590.11617790625239233RNA PCR Primer, Index 1 (100% over 27bp)
CCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG388160.11146003631065672RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG382790.10991804230048507No Hit
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG380340.10921452548020193No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG380240.10918581050794547No Hit
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC369450.10608746500147394RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG369030.10596686211799683No Hit
CCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT368890.10592666115683778RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG366040.10510828444752882No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG365020.10481539173051296No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA362590.1041176179046811No Hit
CCCCCGGGGAGCCCGGCGGGCTGGAATTCTCGGGTGC359980.10336815712878762No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC357810.10274504223082255RNA PCR Primer, Index 1 (100% over 31bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGT354530.10180319114081082No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA350040.10051388888649597No Hit
CCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA349550.10037318552243933RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG348970.1002066386833519No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)