FastQCFastQC Report
Tue 27 Sep 2022
EGAF00004172238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004172238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23568614
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTTCCATCTCGTAT1564610.6638532074902664TruSeq Adapter, Index 1 (97% over 36bp)
AATGATACGGCGACCACCGAGATCTACACGGAGTACTACACTCTTTCCCT447530.18988388540794127Illumina RNA PCR Primer (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTTCCATCTCGTA354730.1505094868964293TruSeq Adapter, Index 1 (97% over 36bp)
CGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTTCCAT293340.1244621342604194TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)