FastQCFastQC Report
Sat 13 May 2023
EGAF00004172248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004172248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27615111
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCACTGATCTCGTAT10108233.6603981059500357TruSeq Adapter, Index 18 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCACTGATCTCGTA7652092.770979265663643TruSeq Adapter, Index 18 (97% over 37bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCACATCCACTGATCTCGTA361380.13086313504226002TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)