FastQCFastQC Report
Thu 2 May 2024
EGAF00004172251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004172251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29137030
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTTGGATCTCGTA5481921.8814271736000545TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTTGGATCTCGTAT2639040.9057340435864601TruSeq Adapter, Index 4 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)