FastQCFastQC Report
Tue 27 Sep 2022
EGAF00004172252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004172252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25632259
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACAGGAATCTCGTAT382334014.9161258084978TruSeq Adapter, Index 4 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACAGGAATCTCGTA9498973.705865331651026TruSeq Adapter, Index 4 (97% over 37bp)
AATGATACGGCGACCACCGAGATCTACACTGACAAGCACACTCTTTCCCT2884001.125144685842945Illumina Single End PCR Primer 1 (96% over 30bp)
GATCGGAATAGCACACGTCTGAACTCCAGTCACTTACAGGAATCTCGTAT681860.2660163507242963TruSeq Adapter, Index 3 (97% over 36bp)
GATCTGAAGAGCACACGTCTGAACTCCAGTCACTTACAGGAATCTCGTAT311530.1215382538074385TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)