FastQCFastQC Report
Wed 15 May 2024
EGAF00004172324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004172324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96273712
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length66
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9219860.9576716019841429No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTCGGCCTTAGCGCCAT3010110.3126616744558473Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2438990.2533391462043138Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCCCTCGACCGCTCGCG2076220.21565803965261043Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2013360.20912873911000754Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTCTGGTCTCGGCCGCA1637640.17010250939529578Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCCCTCGACCGCTCGCGT1568890.1629614115221817Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCTCGGCCTTAGCGCCATT1342200.13941500458609096Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)