FastQCFastQC Report
Fri 24 Mar 2023
EGAF00004172408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004172408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112071655
Sequences flagged as poor quality0
Sequence length66
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11280951.0065836896938838No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGA9609290.8574237616103733Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTG6544230.5839326634375124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3382350.30180244951321544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)