FastQCFastQC Report
Mon 13 May 2024
EGAF00004172410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004172410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110754008
Total Bases7.3 Gbp
Sequences flagged as poor quality0
Sequence length66
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15451171.3950890156498896No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG9710690.8767800078169632Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC6595820.5955378156608111Clontech SMARTer II A Oligonucleotide (100% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3703070.33435087965394444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)