FastQCFastQC Report
Tue 4 Apr 2023
EGAF00004172576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004172576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48964716
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGTCTTTATTTTTCCTCAGCGACTAAAGGCAGCAGCAGTGCCCTGAAG778500.15899203826690225No Hit
ATTTTTCCTCAGCGACTAAAGGCAGCAGCAGTGCCCTGAAGATTAGCAGC772700.15780751184179237No Hit
GTAAAACTGAGGCACGAGCAAAGTGAAGACACTGGCTCATATTCCTGCAG685900.14008046120394121No Hit
TTTTCCTCAGCGACTAAAGGCAGCAGCAGTGCCCTGAAGATTAGCAGCAG556190.11358995730721691No Hit
CTGACACGTCCACCCCAGTGCACCCACTCTGCTTTGACTGAGCAGACTGG530180.10827796897668109No Hit
CTTTATTTTTCCTCAGCGACTAAAGGCAGCAGCAGTGCCCTGAAGATTAG527590.10774901665926134No Hit
GTCCACCCCAGTGCACCCACTCTGCTTTGACTGAGCAGACTGGTGAGCAG499490.102010190358298No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)