FastQCFastQC Report
Sun 14 May 2023
EGAF00004172699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004172699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34379535
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAATGGGAC1828370.5318192930765352No Hit
CCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAG851140.24757170217689098No Hit
CAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAG836450.24329881134227094No Hit
GTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAGCTCCGAGGTGAT766120.22284187380661197No Hit
GAAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCA716240.20833324243623424No Hit
TGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAGCTCCGAGGTGA711390.20692251945816023No Hit
TTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAGCTCCG683940.19893811827297836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA425980.12390510808246825No Hit
CTAGGGTCCACCCCAGGAGGACGGCTGGGGCGGGGACAGGGTCTCCCGCT389600.11332323139332746No Hit
ATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAGCTCC377820.1098967743455518No Hit
CTTTAATTAAGCTAAGCCCTTACTAGACCAATGGGACTTAAACCCACAAA373380.10860530836150054No Hit
CTTAAACCCACAAACACTTAGTTAACAGCTAAGCACCCTAATCAACTGGC367970.10703169778183445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)