FastQCFastQC Report
Sun 14 May 2023
EGAF00004172700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004172700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34379535
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAATGGGAC1735510.5048090382839675No Hit
CAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAG790600.22996238896192167No Hit
GTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAGCTCCGAGGTGAT779720.2267977155595618No Hit
CCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAG763590.22210597089227646No Hit
TGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAGCTCCGAGGTGA718560.20900806249997272No Hit
GAAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCA696160.20249255843629066No Hit
TTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAGCTCCG643180.18708222784281403No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA408590.11884686631160078No Hit
CTAGGGTCCACCCCAGGAGGACGGCTGGGGCGGGGACAGGGTCTCCCGCT391940.11400386887140852No Hit
CTTTAATTAAGCTAAGCCCTTACTAGACCAATGGGACTTAAACCCACAAA375330.10917250625990142No Hit
ATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAGCTCC368260.10711605028980177No Hit
GGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAGCTCCGAG345230.10041729767432864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)